Albert H. Coons

Learn More
Colchicine (CC) enhances the antibody response in mice to protein antigens, like diphtheria toxoid and human gamma globulin, as well as to the 2,4,6-trinitrophenyl hapten. Maximal enhancement was observed when CC was administered to animals on the same day as the injection of antigen. The optimal dose of CC was in the range of 1.0-1.5 mg/kg body weight. The(More)
of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody. A method employing the specificity of an-tibody labeled with fluorescein for the localization of antigen under the fluores-cence microscope is presented. Included in the paper are a description of the synthesis of fluorescein isocyanate,(More)
The simultaneous administration of colchicine (CC) with a T-independent antigen, e.g. 2,4,6-trinitrophenyl-keyhold limpet hemocyanin-Sepharose, to intact animals effectively enhanced their hapten-specific plaque-forming cell (PFC) response. However, in congenitally athymic nude mice in which T-cell regulation was absent, CC was ineffective in producing(More)
Superiority of fluorescein isothiocyan-ate (Riggs) for fluorescent-antibody technic with a modification of its application. The authors compare three methods Cor preparing fluorescein conjugated glob-ulins by using two derivatives of fluo-rescein amine, and recommended one for fluorescent antibody staining. [The SCI ® indicates that this paper was cited a(More)
  • 1