Alastair Nicol

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OBJECTIVE To determine if ice-water immersion after eccentric quadriceps exercise minimises the symptoms of delayed-onset muscle soreness (DOMS). DESIGN A prospective randomised double-blind controlled trial was undertaken. 40 untrained volunteers performed an eccentric loading protocol with their non-dominant leg. INTERVENTIONS Participants were(More)
During asymmetric cytoplasmic mRNA transport, cis-acting localization signals are widely assumed to tether a specific subset of transcripts to motor complexes that have intrinsic directionality. Here we provide evidence that mRNA transcripts control their sorting by regulating the relative activities of opposing motors on microtubules. We show in Drosophila(More)
We present results of experiments on the dynamics of Dictyostelium discoideum in a novel setup which constrains cell motion to a plane. After aggregation, the amoebae collect into round “pancake” structures in which the cells rotate around the center of the pancake. To provide a mechanism for the self-organization of the Dictyostelium cells, we have(More)
T lymphocytes use LFA-1 to migrate into lymph nodes and inflammatory sites. To investigate the mechanisms regulating this migration, we utilize mAbs selective for conformational epitopes as probes for active LFA-1. Expression of the KIM127 epitope, but not the 24 epitope, defines the extended conformation of LFA-1, which has intermediate affinity for ligand(More)
RAS signalling through phosphoinositide 3-kinase (PI3-Kinase) has been shown to have an essential role in tumour initiation and maintenance. RAS also regulates cell motility and tumour invasiveness, but the role of direct RAS binding to PI3-Kinase in this remains uncertain. Here, we provide evidence that disruption of RAS interaction with PI3-Kinase p110α(More)
The inositol lipid phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P2] is involved in a myriad of cellular processes, including the regulation of exocytosis and endocytosis. In this paper, we address the role of PtdIns(4,5)P2 in compound exocytosis from rat peritoneal mast cells. This process involves granule-plasma membrane fusion as well as homotypic(More)
Nonviral gene delivery vectors now show good therapeutic potential: however, detailed characterization of the composition and macromolecular organization of such particles remains a challenge. This paper describes experiments to elucidate the structure of a ternary, targeted, lipopolyplex synthetic vector, the LID complex. This consists of a lipid(More)
Scattered individual amoebae of Dictyostelium discoideum aggregate into multicellular mounds and then sort out to generate distinct prespore and prestalk domains in slug shaped structures (Raper, 1940; Bonner, 1967; Loomis, 1982). The relative simplicity of early development in Dictyostelium makes it an easily accessible model for the study of cell(More)
Studies have been made on muscovite single crystals treated under dry and hydrothermal conditions at temperatures above 800~ The course of reaction is markedly affected by the amount of water present in the system. In the presence of a large excess of water, appreciable reaction commences at 8(~0~ with the formation of kalsilite and corundum. The kalsilite(More)
Activating mutations in Kras are the most frequent mutations in human cancer. They define a subset of patients who do not respond to current therapies and for whom prognosis is poor. Oncogenic Kras has been shown to deregulate numerous signaling pathways of which the most intensively studied are the Ras/extracellular signal-regulated kinase cascade and the(More)