Alan V. Klotz

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Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus.

Randy M. Berka, Michael Willard Rey, Kim M. Brown, Tony S. Byun, Alan V. Klotz
Applied and environmental microbiology
1998

The phyA gene encoding an extracellular phytase from the thermophilic fungus Thermomyces lanuginosus was cloned and heterologously expressed, and the recombinant gene product was biochemically… (More)

An alternative 7-ethoxyresorufin O-deethylase activity assay: a continuous visible spectrophotometric method for measurement of cytochrome P-450 monooxygenase activity.

Alan V. Klotz, J. H. J. Stegeman, Christopher T. Walsh
Analytical biochemistry
1984

A procedure to directly measure the cytochrome P-450-dependent 7-ethoxyresorufin O-deethylase activity with a visible spectrophotometer is described and compared to the standard fluorometric method.… (More)

A possible role for microsomal hexose-6-phosphate dehydrogenase in microsomal electron transport and mixed-function oxygenase activity.

John J. Stegeman, Alan V. Klotz
Biochemical and biophysical research…
1979

Abstract Reduction of cytochrome c and cytochrome P-450 was readily linked to the oxidation of glucose-6-phosphate or galactose-6-phosphate by the enzyme hexose-6-phosphate dehydrogenase in… (More)

A non-specific aminopeptidase from Aspergillus.

Alexander M. Blinkovsky, Tony S. Byun, Kim M. Brown, Elizabeth J. Golightly, Alan V. Klotz
Biochimica et biophysica acta
2000

A fermentation broth supernatant of the Aspergillus oryzae strain ATCC20386 contains aminopeptidase activity that releases a wide variety of amino acids from natural peptides. The supernatant was… (More)

Monoclonal antibodies to liver microsomal cytochrome P-450E of the marine fish Stenotomus chrysops (scup): cross reactivity with 3-methylcholanthrene induced rat cytochrome P-450.

S. S. Park, Holly Jean Miller, Alan V. Klotz, Pamela J. Kloepper-Sams, J. H. J. Stegeman, H. V. Gelboin
Archives of biochemistry and biophysics
1986

Hybridomas were prepared from myeloma cells and spleen cells of BALB/c female mice immunized with hepatic cytochrome P-450E purified from the marine fish, Stenotomus chrysops (scup). Nine independent… (More)

Cytochrome P-450 and monooxygenase activity in cardiac microsomes from the fish Stenotomus chrysops.

J. H. J. Stegeman, Bruce Ray Woodin, Alan V. Klotz, Richard E. Wolke, Nanette Roberts Orme-Johnson
Molecular pharmacology
1982

Cytochrome P-450 isozymes from the marine teleost Stenotomus chrysops: their roles in steroid hydroxylation and the influence of cytochrome b5.

Alan V. Klotz, J. H. J. Stegeman, Bruce Ray Woodin, Elisabeth A. Snowberger, Paul Elbert Thomas, Christopher T. Walsh
Archives of biochemistry and biophysics
1986

Two new cytochrome P-450 forms were purified from liver microsomes of the marine fish Stenotomus chrysops (scup). Cytochrome P-450A (Mr = 52.5K) had a CO-ligated, reduced difference spectrum lambda… (More)

An aryl hydrocarbon hydroxylating hepatic cytochrome P-450 from the marine fish Stenotomus chrysops.

Alan V. Klotz, J. H. J. Stegeman, Christopher T. Walsh
Archives of biochemistry and biophysics
1983

Hepatic microsomal cytochrome P-450 from the untreated coastal marine fish scup, Stenotomus chrysops, was solubilized and resolved into five fractions by ion-exchange chromatography. The major… (More)

Post-translational methylation of asparaginyl residues. Identification of beta-71 gamma-N-methylasparagine in allophycocyanin.

Alan V. Klotz, Julie A. Leary, Alexander N. Glazer
The Journal of biological chemistry
1986

A novel post-translationally modified residue, gamma-N-methylasparagine, was detected in the beta subunit of Anabaena variabilis allophycocyanin. Structure determination was accomplished by isolating… (More)

Phycobiliprotein-Bilin Linkage Diversity

I. Structural, D-Ring-Linked Phycocyanobilins, +4 authors Alexander N. Glazer
1986

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