Alan V. Klotz

Publications37
h-index20
Citations1,453
Highly Influential Citations35
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An alternative 7-ethoxyresorufin O-deethylase activity assay: a continuous visible spectrophotometric method for measurement of cytochrome P-450 monooxygenase activity.
A procedure to directly measure the cytochrome P-450-dependent 7-ethoxyresorufin O-deethylase activity with a visible spectrophotometer is described and compared to the standard fluorometric method.… Expand
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Molecular Characterization and Expression of a Phytase Gene from the Thermophilic Fungus Thermomyces lanuginosus
ABSTRACT The phyA gene encoding an extracellular phytase from the thermophilic fungus Thermomyces lanuginosus was cloned and heterologously expressed, and the recombinant gene product was… Expand
  • 124
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An aryl hydrocarbon hydroxylating hepatic cytochrome P-450 from the marine fish Stenotomus chrysops.
Hepatic microsomal cytochrome P-450 from the untreated coastal marine fish scup, Stenotomus chrysops, was solubilized and resolved into five fractions by ion-exchange chromatography. The major… Expand
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A possible role for microsomal hexose-6-phosphate dehydrogenase in microsomal electron transport and mixed-function oxygenase activity.
Abstract Reduction of cytochrome c and cytochrome P-450 was readily linked to the oxidation of glucose-6-phosphate or galactose-6-phosphate by the enzyme hexose-6-phosphate dehydrogenase in… Expand
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A non-specific aminopeptidase from Aspergillus.
A fermentation broth supernatant of the Aspergillus oryzae strain ATCC20386 contains aminopeptidase activity that releases a wide variety of amino acids from natural peptides. The supernatant was… Expand
  • 22
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Monoclonal antibodies to liver microsomal cytochrome P-450E of the marine fish Stenotomus chrysops (scup): cross reactivity with 3-methylcholanthrene induced rat cytochrome P-450.
Hybridomas were prepared from myeloma cells and spleen cells of BALB/c female mice immunized with hepatic cytochrome P-450E purified from the marine fish, Stenotomus chrysops (scup). Nine independent… Expand
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Chromopeptides from phycoerythrocyanin. Structure and linkage of the three bilin groups
Phycoerythrocyanin carries two covalently attached phycocyanobilin (PCB) groups on the ..beta.. subunit and a phycobiliviolinoid (PXB) group on the ..cap alpha.. subunit. Three distinct bilipeptides… Expand
  • 71
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Characterization of the bilin attachment sites in R-phycoerythrin.
The amino acid sequence around the sites of attachment of all the bilin prosthetic groups of Gastroclonium coulteri R-phycoerythrin, (alpha beta)6 gamma, have been determined. The sequences of… Expand
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Post-translational methylation of asparaginyl residues. Identification of beta-71 gamma-N-methylasparagine in allophycocyanin.
A novel post-translationally modified residue, gamma-N-methylasparagine, was detected in the beta subunit of Anabaena variabilis allophycocyanin. Structure determination was accomplished by isolating… Expand
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Post-translational methylation of phycobilisomes and oxygen evolution efficiency in cyanobacteria
Abstract A post-translationally methylated asparagine residue, γ-N- methylasparagine (NMA), is found at the β-72 site in many phycobiliproteins. We have examined the effects of asparagine methylation… Expand
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