Alan G. Wiseman

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1. Cytochrome P-450 and the associated components and oxidative activities of a mixed-function oxidase (MFO) system are localized primarily in the microsomes of the digestive gland of molluscs. 2. Cytochrome P-450 and putative cytochrome P-450-catalysed oxidative activities, measured in vitro and/or in vivo, have variously been detected in 23 species of(More)
We derive the gravitational waveform and gravitational-wave energy flux generated by a binary star system of compact objects (neutron stars or black holes), accurate through second post-Newtonian order (O[(v/c)4] ∼ O[(Gm/rc)]) beyond the lowest-order quadrupole approximation. We cast the Einstein equations into the form of a flat-spacetime wave equation(More)
The construction of a three-dimensional molecular model of the fungal form of cytochrome P450 (CYP51) from Saccharomyces cerevisiae, based on homology with the haemoprotein domain of CYP102 from Bacillus megaterium (a unique bacterial P450 of known crystal structure) is described. It is found that the endogenous substrate, lanosterol, can readily occupy the(More)
Wild type cells of the green alga Chlamydomonas reinhardtii can grow in the in the dark by taking up and respiring exogenously supplied acetate. Obligate photoautotrophic (dark dier, dk) mutants of this alga have been selected which grow at near wild type rates in the light, but rapidly die when transferred to darkness because of defects in mitochondrial(More)
Succharomyces cerevisiae (N.C.Y.C. no. 240) was grown from loop at 30°C in shake culture [50rev./min, stroke 5cm (2in)l in a medium containing either 1 or 20% glucose (glucose sterilized separately to avoid interfering breakdown pigments) plus 1 % yeast extract, 0.5 % NaCl and 2 % peptone. Separate experiments were terminated at intervals between 17 and(More)
The rate of gravitational-wave energy loss from inspiralling binary systems of compact objects of arbitrary mass is derived through second post-Newtonian (2PN) order O[(Gm/rc2)2] beyond the quadrupole approximation. The result has been derived by two independent calculations of the (source) multipole moments. The 2PN terms, and in particular the finite mass(More)
GRASP (Gravitational Radiation Analysis & Simulation Package) is a public-domain software tool-kit designed for analysis and simulation of data from gravitational wave detectors. This users manual describes the use and features of this package. Note: an up-to-date version of this manual may be obtained at: http://www.ligo.caltech.edu/LIGO(More)
The yeast Saccharomyces cerevisiae, produces a cytochrome P-450 enzyme with a Soret peak in the reduced-CO difference spectrum at 448 nm. The enzyme purified to homogeneity (88-97% pure on a specific content basis) has a molecular wt. of 55 500 as determined by SDS-PAGE. Amino acid analysis of yeast cytochrome P-448 revealed 407 amino acid residues per(More)
Renewed world interest in enzyme biotechnological industries now derives from the expectation that many new biocatalysts will be created by genetic engineering associated with protein engineering designer techniques, or by chemical modification of existing enzymes by use of protein tailoring methods. The biocatalysts produced are mainly enzymes, abzymes(More)