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A method for measuring the spatial concentration of specific categories of vessels in a vascular network consisting of vessels of several diameters, lengths, and orientations is demonstrated. It is shown that a combination of the mathematical morphology operation, opening, with a linear rotating structuring element (ROSE) and dual feature thresholding can(More)
A system has been developed to facilitate three-dimensional visualizations of tomographic image data. Tomographic techniques yield parallel planes of data at discrete locations; thus, a series of images comprises a three-dimensional database. From this database, a system has been developed to perform three-dimensional calculations, measurements, and(More)
A multilayer processing strategy was developed for the automatic screening of conventionally prepared Papanicolaou smears. The processing stages include image segmentation, feature extraction, object classification and slide classification. Mathematical morphology functions were implemented in hardware with custom-built gate array processors for image(More)
A method is presented for imaging single isolated cell nuclei in 3D, employing computed tomographic image reconstruction. The system uses a scanning objective lens to create an extended depth-of-field (DOF) image similar to a projection or shadowgram. A microfabricated inverted v-groove allows a microcapillary tube to be rotated with sub-micron precision,(More)
The authors detail the design and implementation of HANDX, a model-based computer vision system used in the domain of medical image processing. Given a digitized hand radiograph, HANDX segments out specific bones and measures particular parameters of the bones, without requiring specific characterization of noise variations in background contrast and(More)
The practice of clinical cytology relies on bright-field microscopy using absorption dyes like hematoxylin and eosin in the transmission mode, while the practice of research microscopy relies on fluorescence microscopy in the epi-illumination mode. The optical projection tomography microscope is an optical microscope that can generate 3-D images of single(More)
An optical projection tomography microscope (OPTM) can improve axial resolution by viewing a sample from different perspectives. Here, we report a dual-mode OPTM that can generate 3D images of single cancer cells in both absorption mode and polarization mode. Cancer cells were labeled with hematoxylin for absorption imaging and nanorods for polarization(More)
The Cell-CT is an optical projection tomography microscope (OPTM) developed for high resolution 3D imaging of single cells based on absorption stains and brightfield microscopy. In this study we demonstrate the use of the Cell-CT in multi-color mode for simultaneous imaging of cellular 3D morphology and the 3D distribution of nanoparticle clusters in the(More)