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The side chains of residues Thr299 and Thr301 in the Streptomyces R61 DD-peptidase have been modified by site-directed mutagenesis. These amino acids are part of a beta-strand which forms a wall of the active-site cavity. Thr299 corresponds to the second residue of the Lys-Thr(Ser)-Gly triad, highly conserved in active-site beta-lactamases and(More)
Approximate solutions of the Boltzmann equation for secondary electron emission : results and comparison with experiments. Theoretical descriptions of secondary electron emission induced by electron and ion beams impinging on solids. Approximate solution of the Boltzmann electron transport equation in a semi-infinite medium and surface correction to the(More)
The Tole of lysine-67 in a class C fl-lactamase is mainly electrostatic Receptor-mediated Mn2+ influx in rat hepatocytes: comparison of cells loaded with Fura-2 ester and cells microinjected with Fura-2 salt Cloning of cDNA encoding the bifunctional dehydroquinase-shikimate dehydrogenase of aromatic-amino-acid biosynthesis in Nicotiana tabacum Research(More)
Deacetoxycephalosporin C synthase (DAOCS) from Streptomyces clavuligerus catalyses the oxidative ring expansion of the penicillin nucleus into the nucleus of cephalosporins. The reaction requires dioxygen and 2-oxoglutarate as co-substrates to create a reactive iron-oxygen intermediate from a ferrous iron in the active site. The active enzyme is monomeric(More)
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