Akiyuki Hasegawa

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Japanese common newts (Cynops pyrrhogaster) have high ability to regenerate their injured organs including neural tissues, for example, the neural retina belonging to central nervous system. We attempted to clarify the molecular mechanism underlying the formation of a neural network during newt retina regeneration, and focused on the microtubule dynamics(More)
Automated continuous individual cell transfer is a critical step in single-cell applications using microfluidic devices. Cells must be aspirated gently from a buffer before transferring to operation zone so as not to artificially perturb their biostructures. Vision-based manipulation is a key technique for allowing nondestructive cell transportation. In(More)
Three-dimensional (3D) tissues can be reconstructed by cell sheet technology, and various clinical researches using these constructed tissues have already been initiated to regenerate damaged tissues. While 3D tissues can be easily fabricated by layering cell sheets, the attachment period for cell adhesion between a cell sheet and a culture dish, or(More)
Advances in biology have clarified the details of many phenomena and their results have been applied to many fields. However, in these fields, applications require dexterous and skillful manipulation. Hence, realizing high productivity is still difficult. To solve this problem, assisted cell manipulation and automated systems have been researched and(More)
In this paper, we presented a design for an automated cell supply system that can be used with complex microfluidic applications requiring single cell loading such as the current nuclear transplantation method. The aim of the system is to automatically transfer mammalian donor (∼15μm) or egg (∼100μm) cells one by one from a container to a PDMS micro-channel(More)
In this paper, we propose a sensor for detecting particles flowing in a micro channel fabricated with polydimethylsiloxane (PDMS) chip. A light source placed at one side of a micro channel irradiates the micro channel through plastic optical fibers (POF), and a detector placed at the opposite side of the micro channel receives light transmitted through the(More)
Medical simulator is important to improve the operation skills with training system for medical doctors. In this study, dura mater model of human brain is fabricated to aim in reproduction of 3D structure. To mimic the physical properties of actual dura mater tissue, we propose a dura mater model in which are embedded a fiber sheet in a silicone resin. A(More)
Unlike other vertebrates, teleosts have rod- and cone-specific phosducins (PD-R and PD-C) in the retina. To evaluate the teleost Gbetagamma-PD systems, we isolated cDNAs encoding medaka Gbeta1 and GbetaC, which selectively expressed rods and cones. Immunohistochemical studies showed that the strong reactivity of GbetaC but not PD-C was detected in cone(More)