Akihiko Kusanagi

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Three-dimensionally woven poly(epsilon-caprolactone) (PCL) scaffolds were combined with adult human mesenchymal stem cells (hMSC) to engineer mechanically functional cartilage constructs in vitro. The specific objectives were to: (i) produce PCL scaffolds with cartilage-like mechanical properties, (ii) demonstrate that hMSCs formed cartilage after 21 days(More)
The mode of cytotoxic action of protocatechualdehyde on V79 cells was examined. The cytotoxicity of protocatechualdehyde on V79 cells was time- and cell cycle-dependent. The lethal action is selective for the cells at S phase only. The cells lethally damaged at S phase seemed to complete their DNA synthesis, although the rate of DNA synthesis became lower(More)
In the present study, we examined the cranial nerve foramina of Risso's dolphin (Grampus griseus). There were two distinguishable characters in the cranial nerve foramina compared with terrestrial mammals. One was that the foramen infraorbitale was composed of several holes, but not a single hole. They should therefore be termed foramina infraorbitales. The(More)
Cytotoxicity and cytomorphological changes induced in cultured mammalian cells by the application of benzaldehyde, pyrocatechol and their derivatives were examined. Among the compounds tested, 3,4-dihydroxybenzaldehyde (protocatechualdehyde) and 1,2-dihydroxybenzene (pyrocatechol) produced cytomorphological changes characteristic of specific inhibitors of(More)
The pancreas of a striped hyena (Hyena hyena) was examined by the naked eyes and light microscopy. The distribution of A, B, D and PP cells in the islets of Langerhans was investigated immunohistochemically. The pancreas, located in the mesoduodenum, consisted of three lobes. It was about 420 mm in total length, about 10-30 mm in width and about 10 mm in(More)
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