Ake Sellström

Learn More
In rats treated with sodium cyanide (5-20 mg/kg, ip) dopamine was dose dependently decreased in the striatum within 60 sec. One of the main metabolites of dopamine in the central nervous system, 3-methoxy-4-hydroxyphenylacetic acid (HVA), was decreased in striatum, olfactory tubercle, and hippocampus. However, the oxidatively deaminated metabolite,(More)
Potassium-stimulated [3H]GABA release has been studied with fractions of glial cells, neuronal perikarya and synaptosomes using a superfusion technique. A monotonic increase in the [3H]GABA release was observed when external K+ concentration was raised from 5 to 15 mM. However, there was no further increase in the [3H]GABA release when K+ was raised above(More)
C 1300 neuroblastoma cells were cultured and used to study the effect of sodium dependent taurine transport on the membrane potential. Measuring net accumulation of taurine and the depolarization caused by externally applied taurine, we found both processes become active at an external concentration of taurine of 1 mM or more. Net accumulation had Km of 13(More)
Retinas from 3- and 10-day-old rabbits, and from young (29 days), or adult animals were used to study in parallel the development of synaptic vesicles in amacrine cells and the Ca2+ dependence of the K+-stimulated [3H]gamma-aminobutyrate release from them. Few synaptic vesicles were observed in the amacrine cell processes in retinas from the 3-day-old(More)
The development of the GABAergic system in the chick embryo telencephalon has been studied. Special emphasis was placed on the development of glutamate decarboxylase (GAD) between embryonic day 8 (E8) and E17. The GABA immunoreactivity and neuron-specific enolase expression was detected simultaneously in glutardialdehyde fixed sections, which confirmed that(More)
Cultures from cerebral hemispheres of newborn rats contain the enzymes monoamine oxidase (MAO), catechol-O-methyltransferase (COMT), and gamma-aminobutyric acid alpha-ketoglutarate transaminase (GABA-T). The COMT activity was higher in the cultures than in adult rat cerebral hemispheres. The MAO activity was comparable in the cultures and in the rat(More)
Our efforts have been directed towards characterizing amino acid uptake, metabolism and release in bulk-isolated glia and neuronal perikarya studied in parallel with nerve-endings, especially as it concerns the transmitter amino acids and the participation of glia in the clearing of the synpatic space during impulse conduction. A possible neuromodulator(More)
Primary cultures from rat or mouse brain hemispheres contain predominantly glial cells. These cells accumulated [3H]GABA and showed a specific fluorescence with FITC-labeled anti-S-100 antiserum. Round or elongated cells which reacted positively with antimacrophage antiserum and exhibited phagocytotic activity, were considered mesodermal macrophages. Big(More)
Intracellular cAMP increased 9-fold in cerebral hemisphere primary cultures after incubation with dopamine (10(-4) M). The effect was dose- and time-dependent (10(-6) M-10(-4) M; 2-10 minutes). It was mimicked, to some extent, by the partial agonist apomorphine (10(-5) M-10(-4) M) and antagonized by fluphenazine (10(-5) M-10(-4) M). The elevation of cAMP(More)