Ajay S. Gulati

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We tested the hypothesis that Crohn's disease (CD)-related genetic polymorphisms involved in host innate immunity are associated with shifts in human ileum-associated microbial composition in a cross-sectional analysis of human ileal samples. Sanger sequencing of the bacterial 16S ribosomal RNA (rRNA) gene and 454 sequencing of 16S rRNA gene hypervariable(More)
  • Ajay S. Gulati, Michael T. Shanahan, Janelle C. Arthur, Emily Grossniklaus, Richard J. von Furstenberg, Lieselotte Kreuk +3 others
  • 2012
BACKGROUND Increasing evidence supports the central role of Paneth cells in maintaining intestinal host-microbial homeostasis. However, the direct impact of host genotype on Paneth cell function remains unclear. Here, we characterize key differences in Paneth cell function and intestinal microbial composition in two widely utilized, genetically distinct(More)
  • Michael T Shanahan, Ian M Carroll, Emily Grossniklaus, Andrew White, Richard J von Furstenberg, Roshonda Barner +4 others
  • 2014
OBJECTIVE Although polymorphisms of the NOD2 gene predispose to the development of ileal Crohn's disease, the precise mechanisms of this increased susceptibility remain unclear. Previous work has shown that transcript expression of the Paneth cell (PC) antimicrobial peptides (AMPs) α-defensin 4 and α-defensin-related sequence 10 are selectively decreased in(More)
Culture-independent phylogenetic analysis of 16S ribosomal RNA (rRNA) gene sequences has emerged as an incisive method of profiling bacteria present in a specimen. Currently, multiple techniques are available to enumerate the abundance of bacterial taxa in specimens, including the Sanger sequencing, the ‘next generation’ pyrosequencing, microarrays,(More)
BACKGROUND The Internet has successfully been used for patient-oriented survey research. Internet-based translational research may also be possible. OBJECTIVE Our aim was to study the feasibility of collecting biospecimens from CCFA Partners, an Internet-based inflammatory bowel disease (IBD) cohort. METHODS From August 20, 2013, to January 4, 2014, we(More)
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