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Thiobacillus denitrificans, a species of autotrophic facultative anaerobic bacterium, was found to be capable of oxidizing sulfide into elemental sulfur when nitrate was adopted as its electron acceptor and carbon dioxide as its sole carbon source under anoxic conditions. In this way, sulfur was accumulated extracellularly and nitrate was converted into(More)
ABSTRACT The processes and mechanisms of community assembly and its relationships to community functioning are central issues in ecology. Both deterministic and stochastic factors play important roles in shaping community composition and structure, but the connection between community assembly and ecosystem functioning remains elusive, especially in(More)
The roles of fermentative acidogenic bacteria and sulfate-reducing bacteria (SRB) in lactate degradation and sulfate reduction in a sulfidogenic bioreactor were investigated by traditional chemical monitoring and culture-independent methods. A continuously stirred tank reactor fed with synthetic wastewater containing lactate and SO(2-)(4) at 35 degrees C,(More)
High-rate biological conversion of sulfide and nitrate in synthetic wastewater to, respectively, elemental sulfur (S0) and nitrogen-containing gas (such as N2) was achieved in an expanded granular sludge bed (EGSB) reactor. A novel strategy was adopted to first cultivate mature granules using anaerobic sludge as seed sludge in sulfate-laden medium. The(More)
An expanded granular sludge bed (EGSB) reactor was adopted to incubate bio-granules that could simultaneously convert 4.8 kg-Sm(-3) d(-1) of sulfide in 97% efficiency; 2.6 kg-Nm(-3) d(-1) of nitrate in 92% efficiency; and 2.7 kg-Cm(-3) d(-1) acetate in 95% efficiency. Mass balance calculation of sulfur, nitrogen, and carbon over the EGSB reactor confirmed(More)
Denitrifying sulfide removal (DSR) process simultaneously converts sulfide, nitrate, and chemical oxygen demand from industrial wastewaters to elemental sulfur, nitrogen gas, and carbon dioxide, respectively. This investigation utilizes a dilution-to-extinction approach at 10−2 to 10−6 dilutions to elucidate the correlation between the composition of the(More)
The effects of two typical methanogenic inhibitors [2-bromoethanesulfonate (BES) and chloroform (CHCl3)] on the bacterial populations were investigated using molecular ecological techniques. Terminal restriction fragment length polymorphism analyses (T-RFLP) in combination with clone library showed that both the toxicants not only inhibited methanogenic(More)
Influence of acetate-C/NO3(-)-N/S(2-) ratio to the functional microbial community during the denitrifying sulfide removal process is poorly understood. Here, phylogenetic and functional bacterial community for elemental sulfur (S(0)) recovery and nitrate (NO3(-)) removal were investigated with the switched S(2-)/NO3(-) molar ratio ranged from 5/2 to 5/9.(More)
No comprehensive review on the bioconversion of lignocellulosic biomass to hydrogen is presented. This paper provides an up-to-date review on recent research development in biotechnology-based lignocellulosic biomass-to-H(2) conversion. Bioconversion of lignocellulosic prehydrolysate, hydrolysate or cellulose to hydrogen was discussed in terms of the(More)
This study isolated a total of seven pure cultures from activated sludge that could produce bioflocculants from 1.7% v/v H2SO4 treated hydrolysates of corn stover. The most effective strain amongst the seven isolates was identified as Ochrobactrum ciceri W2. The W2 cells produced biopolymers in logarithm growth phase, peaking at 3.8 g l(-1)in productivity(More)