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During a survey conducted in the grapevine orchards of Himachal Pradesh, variety of symptoms ranging from leaf yellowing, vein greening, reduced leaf size, downward rolling/cup shaped leaves to reduced fruit bearing were observed. Symptomatic leaf samples were collected and analyzed by serological (DAS-ELISA) and molecular methods (RT-PCR, PCR) for viruses(More)
Apple is the major commercial horticulture crop in Himachal Pradesh and other hill states of Jammu & Kashmir, Uttarakhand and some parts of Northeastern states of India. In order to gather data on health status and incidence of virus and virus-like pathogens in apple orchards, survey was conducted in the month of June and September, 2010 in Hatkoti, Rohru,(More)
Chrysanthemums worldwide suffer from a high incidence of infection with chrysanthemum virus B (CVB), a member of the genus Carlavirus, family Betaflexiviridae. Three major lineages or strains of this virus have been found in India, but none have been characterized beyond the genetic variation they display in their coat protein genes. Here, we describe the(More)
A rapid and sensitive two-step RT-PCR protocol for simultaneous detection of major apple viruses, namely Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple scar skin viroid (ASSVd), was developed. Five specific primer pairs were tested and confirmed for these(More)
Cherry necrotic rusty mottle virus (CNRMV) and Cherry virus A (CVA) are important graft transmitted viruses in the family Betaflexiviridae, infecting cherry. It is believed that CVA causes severe symptoms and disease in combination with other stone fruit viruses in both cherry and non-cherry hosts while CNRMV infected tree show reduced growth, significant(More)
Cucumber mosaic virus (CMV) has a wide host range causing severe damage in many important agricultural and ornamental crops. Earlier reports showed the prevalence of CMV subgroup I isolates in India. However, some recent reports point towards increasing incidence of subgroup II isolates in the country. The complete genome of a CMV isolate causing severe(More)
Coat protein (CP) and RNA3 from Prunus necrotic ringspot virus (PNRSV-rose), the most prevalent virus infecting rose in India, were characterized and regions in the coat protein important for self-interaction, during dimer formation were identified. The sequence analysis of CP and partial RNA 3 revealed that the rose isolate of PNRSV in India belongs to(More)
The present study identified natural infection of Apple stem grooving virus in five bamboo species. “Himalayan ‘maggar’ bamboo, widely distributed in the Western Himalayas, is abundantly found in and around the campus of CSIR-IHBT. Some of the plants near the apple germplasm and nursery field at this institute displayed virus-like symptoms such as(More)
Among apple viruses, the present study identified Apple stem grooving virus infection in Bauhinia variegata . CP gene of the isolate clustered with kiwi isolate, however, differing in biological characteristics. Bauhinia variegata, widely distributed in Kangra district (Himachal Pradesh, India), was found to exhibit severe virus-like symptoms, which(More)
Polyclonal rabbit antisera were produced using coat protein of Cymbidium mosaic virus (CymMV) Indian isolate expressed in E. coli as GST fusion. The expressed protein was purified by GST-fusion protein purification kit for use as an immunogen in rabbits. Antisera prepared in this manner reacted in double antibody sandwich enzyme-linked immunosorbent assay(More)