Aida Aleksandrovna Selezneva

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The isolation conditions, composition and certain properties of cholesterol oxidase from the mycelium Actinomyces lavendulae were studied. Selective extraction of the enzyme occurred upon the mycelium extraction by the Tween-80 containing buffer. The specific activity of cholesterol oxidase thus obtained exceeded seven-fold that of the enzyme isolated via(More)
By gel chromatography on Sephadex G-75 it has been demonstrated that the culture fluid of Geotrichum asteroides VKM F-144 contains two lipolytic components, one of which, lipase 1, eluates in the free volume of the column, whereas the second component, lipase 2, eluates in the volume close to that of the egg albumin yield. The paper describes a method for(More)
The effect of pH values on the conformation state of the protein globule and enzyme activity of alpha-amylase isolated from the culture liquid of the fungus Aspergillus terricola was studied. By the method of dispersion of optical rotation, it was demonstrated that together with the disordered structure the alpha-amylase macromolecule in its native form(More)
By gel filtration on Sephadex G-75 it has been demonstrated that the lipolytic complex of Penicillium sp. contains two active components--a high molecular weight (lipase-1) and low molecular weight component (lipase-2). Both components have been isolated by Sephadex gel-filtration and DEAE-cellulose chromatography. It has been shown by discelectrophoresis(More)
A comparative study of properties of lipases of microbial origin has shown that the pH optimum of the enzymes from Geotrichum asteroides and Penicillium sp. is at pH 7.5 and 5.0, respectively. Lipase from Geotrichum asteroides appears more resistant to high temperatures and pH changes than the enzyme from Penicillium sp. Both enzymes split selectively(More)
Streptoverticillium mycoheptinicum, a producer of the antifungal antibiotic mycoheptin, was found to produce an inhibitor of Clostridium perfringens phospholipase C. Dynamics of the enzyme accumulation in the culture liquid filtrate was studied. A technique was developed for purification of the inhibitor, which includes adsorption, selective precipitation,(More)
Results are presented of the studies on the proteolytic enzymes produced by the fungus Aspergillus terricola that comprise the antiinflammatory drug Terrilytin. Terrilytin was shown to be a complex of three proteolytic enzymes that contained amylase as well. Two of the proteases (proteases I and II) were of the group of serine proteases by their(More)