Ai Miyashita

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Uncultured archaeal anaerobic methanotrophs (ANMEs) are known to operate the anaerobic oxidation of methane process, an important sink for the greenhouse gas methane in natural environments. In this study, we designed 16S rRNA gene-specific primers for each of the phylogenetic groups of ANMEs (ANME-1, Guaymas Basin hydrothermal sediment clones group within(More)
A quantitative fluorogenic PCR method for detecting methanogenic and methanotrophic orders was established using a refined primer set for the methyl coenzyme M reductase subunit A gene (mcrA). The method developed was applied to several microbial communities in which diversity and abundance of methanogens or anaerobic methanotrophs (ANMEs) was identified by(More)
Anaerobic oxidation of methane (AOM) in marine sediments is an important global methane sink, but the physiological characteristics of AOM-associated microorganisms remain poorly understood. Here we report the cultivation of an AOM microbial community from deep-sea methane-seep sediment using a continuous-flow bioreactor with polyurethane sponges, called(More)
The process performance of a novel anaerobic down-flow hanging sponge (AnDHS) reactor for the treatment of low strength wastewater was investigated. A lab-scale experiment was conducted in which 300-400 mgCOD L(-1) of artificial wastewater was fed in over 600 days. The reactor exhibited sufficient performance: 70-90% of total COD removal, and 60-90% of(More)
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