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Small interfering RNAs (siRNAs) are the mediators of mRNA degradation in the process of RNA interference (RNAi). Here, we describe a human biochemical system that recapitulates siRNA-mediated target RNA degradation. By using affinity-tagged siRNAs, we demonstrate that a single-stranded siRNA resides in the RNA-induced silencing complex (RISC) together with(More)
single-stranded RNA should bind fairly readily , opening the claw of the molecule might assist binding the mRNA, after which Argo-naute might close on the double-stranded sub-strate. A possible hinge region exists in the interdomain connector at residues 317 to 320. This hinge could lift the PAZ away from the crescent base, perhaps allowing the RISC loading(More)
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