Agnieszka Nowacka

Learn More
Spectral editing with CP and INEPT in (13)C MAS NMR enables identification of rigid and mobile molecular segments in concentrated assemblies of surfactants, lipids, and/or proteins. In order to get stricter definitions of the terms "rigid" and "mobile", as well as resolving some ambiguities in the interpretation of CP and INEPT data, we have developed a(More)
A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With(More)
Amyloid deposits from several human diseases have been found to contain membrane lipids. Co-aggregation of lipids and amyloid proteins in amyloid aggregates, and the related extraction of lipids from cellular membranes, can influence structure and function in both the membrane and the formed amyloid deposit. Co-aggregation can therefore have important(More)
The phase behavior of amphiphiles, e.g., lipids and surfactants, at low water content is of great interest for many technical and pharmaceutical applications. When put in contact with air having a moderate relative humidity, amphiphiles often exhibit coexistence between solid and liquid crystalline phases, making their complete characterization difficult.(More)
The stratum corneum (SC) is an effective permeability barrier. One strategy to increase drug delivery across skin is to increase the hydration. A detailed description of how hydration affects skin permeability requires characterization of both macroscopic and molecular properties and how they respond to hydration. We explore this issue by performing(More)
Despite the enormous potential for pharmaceutical applications, there is still a lack of understanding of the molecular details that can contribute to increased permeability of the stratum corneum (SC). To investigate the influence of hydration and heating on the SC, we record the natural-abundance (13)C signal of SC using polarization transfer solid-state(More)
The outermost layer of the skin, the stratum corneum (SC), is a lipid-protein membrane that experiences considerable osmotic stress from a dry and cold climate. The natural moisturizing factor (NMF) comprises small and polar substances, which like osmolytes can protect living systems from osmotic stress. NMF is commonly claimed to increase the water content(More)
Numerous experimental studies showed that estrogen alters diameters of cerebral arteries by modifying production of vasoactive substances. In this study, we address a question whether increased concentration of 17-beta-estradiol (E2) during a typical menstrual cycle of young, healthy women influences cerebrovascular impedance, as measured with Doppler(More)
We present a short sketch of how the knowledge of thyroid surgery has evolved throughout decades with pointing out to prominent surgeons who achieved great deeds in the history of thyroidectomy. The progress of thyroid surgery is believed to be of utmost importance because it reflects evolution of surgery itself. Although surgeons of our days can rely on(More)