Agnieszka Mikłosz

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AIMS To determine the presence and possible involvement of FAT/CD36, FABPpm and FATP-2, transporters in (i) fatty acids movement across plasma membrane and (ii) an induction of insulin resistance by palmitic (PA) and oleic (OA) fatty acids in primary hepatocytes. METHODS Primary hepatocytes were treated with either PA and OA or combination of activators(More)
Nowadays wrong nutritional habits and lack of physical activity give a rich soil for the development of insulin resistance and obesity. Many researches indicate lipids, especially the one from the sphingolipids class, as the group of molecules heavily implicated in the progress of insulin resistance in skeletal muscle. Recently, scientists have focused(More)
BACKGROUND The objective of this study was to examine the effects of short (2 h) and prolonged (18 h) inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate (PA) induced insulin resistance in L6 myotubes. METHODS L6 myotubes were treated simultaneously with either PA and myriocin (SPT inhibitor) or PA and Ski II(More)
Thyroid hormones (T3, T4) are well known modulators of different cellular signals including the sphingomyelin pathway. However, studies regarding downstream effects of T3 on sphingolipid metabolism in skeletal muscle are scarce. In the present work we sought to investigate the effects of hyperthyroidism on the activity of the key enzymes of ceramide(More)
Insulin resistance (IR) is commonly defined as a lack of insulin effects on target tissues, due to impaired post-receptor signaling pathways. Generally, liver IR is manifested by uncontrolled glucose release to the blood stream (hyperglycemia). However, metabolic consequences of hepatic insulin resistance are more profound, involving also lipid imbalances.(More)
Diabetes is considered a major public health problem affecting millions of individuals worldwide. Remarkably, scientific reports regarding salivary glands sphingolipid metabolism in diabetes are virtually non-existent. This is odd given the well-established link between the both in other tissues (e.g., skeletal muscles, liver) and the key role of these(More)
Skeletal muscle plays an essential role in the regulation of whole-body glucose homeostasis. Glucose is transported into the muscle cells via protein-mediated transport that requires sarcolemmal glucose transporters (GLUT). Translocation of GLUT-4 to the plasma membranes is the most potent factor stimulating glucose uptake by myocytes. Relocation of GLUT-4(More)
Currently, obesity is a predominant medical condition and an important risk factor for the development of several diseases, including type 2 diabetes mellitus. Importantly, most research has indicated lipid-induced insulin resistance in skeletal muscles is a key link between the aforementioned pathological conditions. PGC-1α is a prominent regulator of(More)
BACKGROUND/AIMS AS160 is a key intracellular regulator of energy utilization in cells. It was shown to regulate GLUT4 translocation from intracellular depots to the plasma membrane, with subsequent changes in facilitated glucose uptake into the skeletal muscles. Similarly, also free fatty acids (FFAs) transmembrane transport seems to be largely(More)
BACKGROUND Thyroid hormones (THs) are key regulators of cardiac physiology as well as modulators of different cellular signals including the sphingomyelin/ceramide pathway. The objective of this study was to examine the effect of hyperthyroidism on the metabolism of sphingolipids in the muscle heart. METHODS Male Wistar rats were treated for 10 days with(More)