Adriana M. Alippi

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Ninety-nine strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease (AFB) of honeybees, were isolated from different regions of Argentina and other countries. The isolates were characterized on the basis of DNA fingerprints by a polymerase chain reaction technique (PCR) with BOX sequence-specific primers. Isolates from(More)
A semi-selective medium for the detection in Argentinian honeys of spores of Bacillus larvae, a pathogen of American foulbrood, was developed. The technique involves dilution of samples (1:2) in phosphate buffer, concentration of spores by centrifugation and heat treatment prior to inoculation. Two media (JNxPa and JNxPb) were prepared from J-agar, to which(More)
A rapid procedure for the identification of Paenibacillus larvae subsp. larvae, the causal agent of American foulbrood (AFB) disease of honeybees (Apis mellifera L.), based on PCR and restriction fragment analysis of the 16S rRNA genes (rDNA) is described. Eighty-six bacterial strains belonging to 39 species of the genera Paenibacillus, Bacillus,(More)
Paenibacillus larvae is the causal agent of American Foulbrood (AFB) disease, the most virulent bacterial disease of honeybee (Apis mellifera L.) brood. Oxytetracycline is the main antibiotic used for prevention and control of AFB. Using the polymerase chain reaction, isolates were screened for the presence of the tetracycline resistance tet(K) and tet(L)(More)
AIMS A reliable procedure for the identification of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease of honey bees (Apis mellifera L.) based on the polymerase chain reaction (PCR) and subspecies - specific primers is described. METHODS AND RESULTS By using ERIC-PCR, an amplicon of ca 970 bp was found among P. l. larvae(More)
Of 433 honey samples collected in Argentina, 114 (27%) yielded Bacillus cereus isolates (27%) and 60 (14%) yielded other species of Bacillus. The Argentinian B. cereus isolates were compared with isolates recovered from honey from other countries (n=16) and with strain ATCC 11718. The 133 B. cereus isolates and the ATCC strain were characterized using(More)
One hundred and thirty two Bacillus cereus and 52 Bacillus megaterium isolates from honeys were evaluated for the presence of genes encoding enterotoxin HBL, enterotoxin-T, cytotoxin K and the NHE complex, respectively. The relationship between hemolytic and coagulase activity and its correlation with the presence of the four mentioned enterotoxins was(More)
The present study was conducted to determine whether Varroa jacobsoni can transmit American foulbrood (AFB), caused by the bacterium Paenibacillus larvae to healthy colonies by the surface transport of spores. Five two-storey Langstroth colonies of Apis mellifera ligustica were infested by placing a sealed brood comb, with 10% Varroa prevalence, between the(More)
A collection of 91 isolates from different geographical origins of Paenibacillus larvae, the etiologic agent of American Foulbrood disease of honey bees, was characterized according to its biochemical type and susceptibility to oxytetracycline hydrochloride (OTC), the most commonly used antibiotic for the control of the disease. The majority of the(More)
American foulbrood caused by Bacillus larvae White is recorded for the first time on brood combs of Argentinian hives. The identification of the causative agent was based on disease symptomatology, morphological characters, pathogenicity tests and physiological and biochemical reactions. Studies by scanning electron microscopy showed the occurrence of large(More)