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We recently used in situ Hi-C to create kilobase-resolution 3D maps of mammalian genomes. Here, we combine these maps with new Hi-C, microscopy, and genome-editing experiments to study the physical structure of chromatin fibers, domains, and loops. We find that the observed contact domains are inconsistent with the equilibrium state for an ordinary(More)
Activation of the μ-opioid receptor (μOR) is responsible for the efficacy of the most effective analgesics. To shed light on the structural basis for μOR activation, here we report a 2.1 Å X-ray crystal structure of the murine μOR bound to the morphinan agonist BU72 and a G protein mimetic camelid antibody fragment. The BU72-stabilized changes in the μOR(More)
Author Contributions: W.H. developed functional purification protocols, expressed and purified μOR, characterized the effect of nanobodies and G i on μOR ligand affinity, identified Nb39 for crystallography of the μOR-Nb complex, performed crystallization trials, data collection, structure determination and refinement. A.M. established the project with(More)
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