Aditya Radhakrishnan

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A major determinant of mRNA half-life is the codon-dependent rate of translational elongation. How the processes of translational elongation and mRNA decay communicate is unclear. Here, we establish that the DEAD-box protein Dhh1p is a sensor of codon optimality that targets an mRNA for decay. First, we find mRNAs whose translation elongation rate is slowed(More)
Protein output from synonymous codons is thought to be equivalent if appropriate tRNAs are sufficiently abundant. Here we show that mRNAs encoding iterated lysine codons, AAA or AAG, differentially impact protein synthesis: insertion of iterated AAA codons into an ORF diminishes protein expression more than insertion of synonymous AAG codons. Kinetic(More)
Gene expression and regulation in organisms minimally depends on transcription by RNA polymerase and on the stability of the RNA product (for both coding and non-coding RNAs). For coding RNAs, gene expression is further influenced by the amount of translation by the ribosome and by the stability of the protein product. The stabilities of these two classes(More)
12 Protein output from synonymous codons is thought to be equivalent if appropriate 13 tRNAs are sufficiently abundant. Here we show that mRNAs encoding iterated lysine codons, 14 AAA or AAG, differentially impact protein synthesis: insertion of iterated AAA codons into an 15 ORF diminishes protein expression more than insertion of synonymous AAG codons.(More)
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