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Periplasmic permeases are composed of four proteins, one of which has an ATP-binding site that has been postulated to be involved in energy coupling. Previous data suggested that these permeases derive energy from substrate level phosphorylation (Berger, E. A. (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 1514-1518); however, conflicting results later cast doubt(More)
Bacterial periplasmic binding proteins are initial receptors in the process of active transport across cell membranes and/or chemotaxis. Among them, the histidine-binding protein (HisJ) has been extensively studied from the biochemical, physiological, and genetic points of view. The three-dimensional crystal structure of the histidine-binding protein(More)
Conversion of benzaldehyde to l-phenylacetyl carbinol (l-PAC) was achieved with immobilized, growing cells of Saccharomyces cerevisiae in different reactors. Product formation increased (31%) with the subsequent initial reuses of the entrapped cells. Biomass production and PAC formation depleted (40 and 57%, respectively) after 4–5 continuous growth and(More)
The histidine-binding protein, HisJ, is the soluble receptor for the periplasmic histidine permease of Salmonella typhimurium. The receptor binds the substrate in the periplasm, interacts with the membrane-bound complex, transmits a transmembrane signal to hydrolyze ATP, and releases the ligand for translocation. HisJ, like other periplasmic receptors, has(More)
The periplasmic histidine-binding protein, HisJ, is a receptor for the histidine permease of Salmonella typhimurium. Receptors of this type are composed of two lobes that are far apart in the unliganded structure (open conformation) and drawn close together in the liganded structure (closed conformation). The binding of the ligand, in a cleft between the(More)
The oomycete Lagenidium giganteum, a facultative parasite of mosquito larvae requires exogenous sterols for the genesis of zoospores when grown saprobically. Growth media prepared from oil rich materials such as soy or sunflower seed were very effective inducers of virulent zoospores. The external morphology of zoospores of L. giganteum was studied with the(More)
For any country, the forest policy is an important guideline to maintain forest resources and their interaction with other land uses. India devised its first National Forest Policy (NFP) back in 1894. There has been a paradigm shift from timber production to forest conservation followed by community-based agroforestry and social forestry bringing a change(More)
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