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Determination of the sites of tyrosine O-sulfation in peptides and proteins
TLDR
This methodology identified the sites and the order of sulfation of several peptides mediated by purified human tyrosylprotein sulfotransferases (TPSTs), and unambiguously determined the tyrosine sulfation sites in mouse lumican and human vitronectin. Expand
Detection and Purification of Tyrosine-sulfated Proteins Using a Novel Anti-sulfotyrosine Monoclonal Antibody*
TLDR
Western blot analysis using PSG2 showed that certain sperm/epididymal proteins are undersulfated in Tpst2-/- mice, which indicates that TPST1 and TPST2 have distinct macromolecular substrate specificities and provides clues as to the molecular mechanism of the infertility of T pst 2-/- males. Expand
The discovery of aspirin's antithrombotic effects.
TLDR
The story of Lawrence Craven's story is presented, which demonstrates the value of a single physician's commitment to lifelong learning and the work of the physicians and scientists who discovered the molecular mechanisms by which aspirin exerts its antiplatelet effects. Expand
Tyrosylprotein Sulfotransferase-2 Expression Is Required for Sulfation of RNase 9 and Mfge8 in Vivo*
TLDR
It is demonstrated that RNase 9 and Mfge8 are tyrosine-sulfated in wild type and Tpst1-/-, but not in T pst2-/- mice, which suggests that lack of sulfation of one or both of these proteins may contribute mechanistically to the infertility of TpSt2- +/- males. Expand
Mass spectrometric kinetic analysis of human tyrosylprotein sulfotransferase-1 and -2
TLDR
A liquid chromatography electrospray ionisation mass spectrometry (LC/ESI-MS)-based TPST assay was developed to determine the kinetic parameters of individual TPSTs and a mixture of both isozymes using CCR8 peptides as substrates that have three tyrosine residues in series. Expand
Early postnatal pulmonary failure and primary hypothyroidism in mice with combined TPST-1 and TPST-2 deficiency.
TLDR
Tpst1 and T pst2 are the only Tpst genes in mice, tyrosine sulfation is required for normal pulmonary function at birth, and TPST-2 is needed for normal thyroid gland function, suggesting that a protein(s) required for thyroid hormone production is sulfated and cannot be sulfated in the absence of TP ST-2. Expand
Pretreatment with troglitazone decreases lethality during endotoxemia in mice
TLDR
The results of these studies suggest that troglitazone treatment protects mice against a lethal challenge of LPS, but whether or not this effect is mediated through decreased expression of inflammatory mediators remains unclear. Expand
Protein tyrosine-O-sulfation in the retina.
TLDR
Data show that retinal protein tyrosine-O-sulfation is highly conserved which suggest a functional significance of these proteins to retinal function and structure. Expand
Differential Developmental Deficits in Retinal Function in the Absence of either Protein Tyrosine Sulfotransferase-1 or -2
TLDR
The results demonstrate the functional importance of protein-tyrosine sulfation for proper development of the retina and suggest that the different phenotypes resulting from elimination of either TPST-1 or -2 may reflect differential expression patterns or levels of the enzymes. Expand
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