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Though many individual transcription factors are known to regulate hematopoietic differentiation, major aspects of the global architecture of hematopoiesis remain unknown. Here, we profiled gene expression in 38 distinct purified populations of human hematopoietic cells and used probabilistic models of gene expression and analysis of cis-elements in gene(More)
Increasing demand for human hematopoietic stem cells (HSCs) in clinical and research applications necessitates expansion of HSCs in vitro. Before these cells can be used they must be carefully evaluated to assess their stem cell activity. Here, we expanded cord blood CD34(+) CD133(+) cells in a defined medium containing angiopoietin like 5 and insulin-like(More)
In this paper, we consider a sentiment regression problem: summarizing the overall sentiment of a review with a real-valued score. Empirical results on a set of labeled reviews show that real-valued sentiment modeling is feasible, as several algorithms improve upon baseline performance. We also analyze performance as the granularity of the classification(More)
Therapy-resistant microenvironments represent a major barrier toward effective elimination of disseminated malignancies. Here, we show that select microenvironments can underlie resistance to antibody-based therapy. Using a humanized model of treatment refractory B cell leukemia, we find that infiltration of leukemia cells into the bone marrow rewires the(More)
Preface This is the 23rd edition of the Haskell Communities and Activities Report. As usual, fresh entries are formatted using a blue background, while updated entries have a header with a blue background. Entries for which I received a liveness ping, but which have seen no essential update for a while, have been replaced with online pointers to previous(More)
Many learning algorithms attempt, either explicitly or implicitly, to discover useful high-order features. When considering all possible functions that could be encountered, no particular type of high-order feature should be more useful than any other. However, this paper presents arguments and empirical results that suggest that for the learning problems(More)
How tumor-infiltrating lymphocytes (TILs) that are tumor-specific but functionally tolerant persist in the antigen-expressing tumor tissue is largely unknown. We have previously developed a modified TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model where prostate cancer cells express the T-cell epitope SIYRYYGL (SIY) recognized by CD8 T cells(More)
T lymphocytes require signals from self-peptides and cytokines, most notably interleukins 7 and 15 (IL-7, IL-15), for survival. While mouse T cells die rapidly if IL-7 or IL-15 is withdrawn, human T cells can survive prolonged withdrawal of IL-7 and IL-15. Here we show that IL-7 and IL-15 are required to maintain human T cell proliferative capacity through(More)