Adam C Miller

Learn More
The nematode Caenorhabditis elegans migrates toward a preferred temperature on a thermal gradient. A candidate neural network for thermotaxis in C. elegans has been identified, but the behavioral strategy implemented by this network is poorly understood. In this study, we tested whether thermal migration is achieved by modulating the probability of turning(More)
Lateral inhibition mediated by Delta/Notch (Dl/N) signaling is used throughout development to limit the number of initially equivalent cells that adopt a particular fate. Although adjacent cells express both Dl ligand and N receptor, signaling between them ultimately occurs in only one direction. Classically, this has been explained entirely by feedback:(More)
Recent evidence suggests that stochasticism is important for generating cell type diversity. We have identified a novel stochastic fate choice as part of the mechanism by which Delta/Notch (Dl/N) signaling specifies R7 fate in the Drosophila eye. The equivalence of R1/R6/R7 precursors is normally broken by the activation of N, which specifies the R7 fate.(More)
Karyotypic study of a population sample of twenty-one owl monkeys, Aotus, originating from Bolivia, revealed two sex-specific somatic diploid chromosome numbers: 49 for males and 50 for females. The presence of a trivalent in male meiotic figures and the identification of chromosomes by band pattern analysis confirmed the interpretation of a Y-autosome(More)
Identifying genes involved in biological processes is critical for understanding the molecular building blocks of life. We used engineered CRISPR (clustered regularly interspaced short palindromic repeats) to efficiently mutate specific loci in zebrafish (Danio rerio) and screen for genes involved in vertebrate biological processes. We found that increasing(More)
BACKGROUND Neural networks and their function are defined by synapses, which are adhesions specialized for intercellular communication that can be either chemical or electrical. At chemical synapses, transmission between neurons is mediated by neurotransmitters, whereas at electrical synapses, direct ionic and metabolic coupling occur via gap junctions(More)
Diploid numbers, chromosome morphology, G- and C-banding characterisitics and pelage phenotypes were studied in 330 owl monkeys (Aotus) captured and exported from several parts of South America. Among these animals, seven distinctive karyotypes were recognized by the number of chromosomes and their individual identification by G- and C-banding methods.(More)
Forward genetic screens have elucidated molecular pathways required for innumerable aspects of life; however, identifying the causal mutations from such screens has long been the bottleneck in the process, particularly in vertebrates. We have developed an RNA-seq-based approach that identifies both the region of the genome linked to a mutation and candidate(More)
The sensorimotor transformation underlying Caenorhabditis elegans chemotaxis has been difficult to measure directly under normal assay conditions. Thus, key features of this transformation remain obscure, such as its time course and dependence on stimulus amplitude. Here, we present a comprehensive characterization of the transformation as obtained by(More)