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Gene arrays containing all currently known open reading frames of Bacillus subtilis were used to examine the general stress response of Bacillus. By proteomics, transcriptional analysis, transposon mutagenesis, and consensus promoter-based screening, 75 genes had previously been described as sigma(B)-dependent general stress genes. The present gene(More)
OBJECTIVE Evidence from basic neurophysiology and molecular genetics has implicated persistent sodium current conducted by voltage-gated sodium (NaV ) channels as a contributor to the pathogenesis of epilepsy. Many antiepileptic drugs target NaV channels and modulate neuronal excitability, mainly by a use-dependent block of transient sodium current,(More)
A consensus-directed search for sigma(B) promoters was used to locate potential candidates for new sigma(B)-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations with total RNA from exponentially growing or ethanol-stressed cells of the wild type as well as a sigB mutant revealed 22 genes that required(More)
Bacillus subtilis responds to various stimuli (heat, ethanol and salt stress, energy starvation) with the induction of general stress proteins (GSPs). Most of them belong to the stress and stationary-phase regulon controlled by the alternative sigma factor sigmaB. The majority of sigmaB-dependent proteins are thought to provide a precautionary general(More)
The gene katX, which encodes a catalase in Bacillus subtilis, is transcribed by EsigmaF in the pre-spore. This catalase is responsible for the resistance to hydrogen peroxide shown by germinating and outgrowing spores. We demonstrated that katX is also a sigmaB-dependent general stress gene, since it is strongly induced by heat, salt and ethanol stress, as(More)
The gene katX, which encodes a catalase in Bacillus subtilis, is transcribed by EσF in the pre-spore. This catalase is responsible for the resistance to hydrogen peroxide shown by germinating and outgrowing spores. We demonstrated that katX is also a σB-dependent general stress gene, since it is strongly induced by heat, salt and ethanol stress, as well as(More)
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