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Human dermal fibroblasts obtained by skin biopsy can be reprogrammed directly to pluripotency by the ectopic expression of defined transcription factors. Here, we describe the derivation of induced pluripotent stem cells from CD34+ mobilized human peripheral blood cells using retroviral transduction of OCT4/SOX2/KLF4/MYC. Blood-derived human induced(More)
In embryonic stem cell (ESC) lines generated from human embryos determined through preimplantation genetic diagnosis to carry the fragile X mutation, the FMR1 gene is expressed in undifferenti-ated cells but undergoes transcriptional silencing after ESC differentiation (Eiges et al., 2007). Here, we generated induced pluripotent stem cell (iPSC) lines from(More)
The let-7 tumor suppressor microRNAs are known for their regulation of oncogenes, while the RNA-binding proteins Lin28a/b promote malignancy by inhibiting let-7 biogenesis. We have uncovered unexpected roles for the Lin28/let-7 pathway in regulating metabolism. When overexpressed in mice, both Lin28a and LIN28B promote an insulin-sensitized state that(More)
Supplemental Experimental Procedures Production of virus. Cell culture and human iPS cell induction. Donor blood cells collection and isolation. Immunohistochemistry staining. qRT–PCR. Global gene expression. Bisulfite genomic sequencing. DNA fingerprinting and karyotyping. Embryoid body formation. Hematopoietic colony-forming assays. Assay for teratoma(More)
Turner's syndrome (caused by monosomy of chromosome X) is one of the most common chromosomal abnormalities in females. Although 3% of all pregnancies start with XO embryos, 99% of these pregnancies terminate spontaneously during the first trimester. The common genetic explanation for the early lethality of monosomy X embryos, as well as the phenotype of(More)
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