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In order to estimate the level of oxidative damage and its role in breast cancer, the promutagenic oxidative lesion, 8-hydroxy-2'-deoxyguanosine (8-OHdG), was determined in DNA isolated from 75 human breast tissue specimens and from normal and transformed human breast cell lines, utilising a newly developed solid-phase immunoslot blot assay. The amount of(More)
Nucleotide excision repair (NER), the most versatile and ubiquitous mechanism for DNA repair, operates to remove many types of DNA base lesions. We have studied the role of p53 function in modulating the repair of DNA damage following UV irradiation in normal and p53-compromised human mammary epithelial cells (HMEC). The effect of UV-induced DNA damage on(More)
Primary spinal primitive neuroectodermal tumor (PSPNET) is extremely rare and only 25 cases have been reported in the world literature so far. Three patients of 8, 9 and 18 years of age, who presented with variable grades of neurological deficit were diagnosed as having a dorsal intramedullary lesion, a holocord lesion and cervical extradural tumor with(More)
AIMS The aim of the study was to compare the effect of mannitol (M) and hypertonic saline (HTS) on brain relaxation and electrolyte balance. SETTINGS AND DESIGN Prospective, randomized, double-blind study. SUBJECTS AND METHODS A total of 114 patients with American Society of Anesthesiologists status II and III, scheduled to undergo craniotomy for(More)
Five unusual cases of posterior skull base tumors were treated through different skull base approaches. Two or more staged operations were required to achieve total or near-total excision and decompression of two extensive tumors. Total excision of an extensive en plaque meningioma of the foramen magnum that encircled the brain stem and cervical spinal cord(More)
Fine analysis of DNA damage and repair at the subgenomic level has indicated a microheterogeneity of DNA repair in mammalian cells, including human. In addition to the well established Southern hybridization-based approach to investigate gene-specific DNA damage and repair, alternative methods utilizing the sensitivity of PCR have been evaluated. The latter(More)
Neurocytomas are rare neoplasms, composed of uniform round cells with neuronal differentiation. We report a rare extraventricular neurocytoma (EVN) in a 5-year-old boy who presented with headache and vomiting. His MRI brain showed a vermian mass. The histopathological examination and immunohistochemistry revealed it to be an EVN. We report this patient for(More)
The modified DNA base 8-hydroxyguanine has been implicated in spontaneous mutagenesis, carcinogenesis and cellular aging. Polyclonal antibodies specific for the 8-hydroxy-2'-deoxyguanosine moiety in oxidized DNA were used for sensitive detection and quantitation of this biomarker of oxidative damage to cellular DNA. The analysis was performed with(More)