Abraham M. Rosenbaum

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Genome sequencing of large numbers of individuals promises to advance the understanding, treatment, and prevention of human diseases, among other applications. We describe a genome sequencing platform that achieves efficient imaging and low reagent consumption with combinatorial probe anchor ligation chemistry to independently assay each base from patterned(More)
We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. We apply this technology to resequence an evolved strain of Escherichia coli at less than one error per million consensus bases. A cell-free, mate-paired library provided single DNA(More)
Untreated (–) and ExoSAP-IT treated (+) PCR products were analyzed by gel electrophoresis. A variety of PCR products of different lengths may be treated with ExoSAP-IT, with no sample loss. 125 bp 455 bp 1.55 kb 4.6 kb HES-1 numb NRAGE numb M – + – + – + – + Stop losing time and samples. ExoSAP-IT ® offers quick, one step PCR clean-up with 100% recovery of(More)
Rapid advances in DNA sequencing promise to enable new diagnostics and individualized therapies. Achieving personalized medicine, however, will require extensive research on highly reidentifiable, integrated datasets of genomic and health information. To assist with this, participants in the Personal Genome Project choose to forgo privacy via our(More)
Utilizing the full power of next-generation sequencing often requires the ability to perform large-scale multiplex enrichment of many specific genomic loci in multiple samples. Several technologies have been recently developed but await substantial improvements. We report the 10,000-fold improvement of a previously developed padlock-based approach, and(More)
Center for Inherited Cardiovascular Disease, Division of Cardiovascular Medicine (E A Ashley MRCP, M T Wheeler MD, F E Dewey MD, J W Knowles MD, A Pavlovic BS), Department of Medicine (Prof R B Altman MD), Department of Bioengineering (S R Quake PhD, D Pushkarev, N F Neff PhD, Prof R B Altman), Division of Medical Genetics (Prof L Hudgins MD), Department of(More)
Radoje Drmanac,* Andrew B. Sparks, Matthew J. Callow, Aaron L. Halpern, Norman L. Burns, Bahram G. Kermani, Paolo Carnevali, Igor Nazarenko, Geoffrey B. Nilsen, George Yeung, Fredrik Dahl, Andres Fernandez, Bryan Staker, Krishna P. Pant, Jonathan Baccash, Adam P. Borcherding, Anushka Brownley, Ryan Cedeno, Linsu Chen, Dan Chernikoff, Alex Cheung, Razvan(More)
DNA sequencing advancements in cost and throughput are under intensive development to enable widespread discovery of genomics information. Some groups have focused on conventional dideoxy sequencing, making improvements that include performing separations in microchannels [1], while others have shifted to using cyclic array sequencing technologies [2]. Our(More)
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