Abigael Brownell

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Low molecular mass amelogenin-related polypeptides extracted from mineralized dentin have the ability to affect the differentiation pathway of embryonic muscle fibroblasts in culture and lead to the formation of mineralized matrix in in vivo implants. The objective of the present study was to determine whether the bioactive peptides could have been(More)
Numerous investigations have demonstrated the necessity of mesenchymal instruction for epithelial differentiation during epidermal organogenesis. In the specific case of tooth formation, cap-stage tooth organ mesenchyme instructs epithelial differentiation into ameloblasts with production of enamel extracellular matrix. The "instructive event" is presumed(More)
To explore the modulatory effects of IL-2-activated NK cells on hematopoietic stem cell (HSC) engraftment further, we used fresh newborn liver cells (NLC) and IL-2-activated newborn liver cells (ANLC) as combined sources, respectively, of transplanted HSC and IL-2-activated NK cells free of contaminating CD3+ T cells. As previously found with adult(More)
Bovine bone morphogenetic protein (bBMP) induces differentiation of mesenchymal-type cells into cartilage and bone. bBMP has an apparent Mr of 18,500 +/- 500 and represents less than 0.001% of the wet weight of bone tissue. A Mr 34,000 protein resembling osteonectin is separated by extraction with Triton X-100. A Mr 24,000 protein and about half of a Mr(More)
Human bone morphogenetic protein (hBMP) was chemically extracted from demineralized gelatinized cortical bone matrix by means of a CaCl2 X urea inorganic-organic solvent mixture, differential precipitation in guanidine hydrochloride, and preparative gel electrophoresis. hBMP is isolated in quantities of 1 mg/kg of wet weight of fresh bone, and has the(More)
Epithelial synthesis and secretion of basal lamina has been considered to be a general feature of various vertebrate epithelium-mesenchyme interacting systems (e.g., salivary gland, mammary gland, feather, hair, and tooth morphogenesis). It has been repeatedly assumed that embryonic ectoderm and ectodermal derivatives, such as epithelial tissues associated(More)
Fibronectin is a large molecular weight glycoprotein which has been shown to be associated with cell surfaces, extracellular fluids, and connective tissues. Its possible relationship with basement membranes remains controversial. To define this relationship, the distribution of this antigen was evaluated by light microscopic immunoperoxidase techniques in(More)
Fibronectin, basement membrane and type I collagen antigens have been localized in normal rat kidney by electron immunohistochemical methods. Immunoreactive fibronectin was found in the interstitial connective tissue matrix and on collagen fibers, while tubular, endothelial and smooth muscle basement membranes throughout the kidney were consistently(More)
It has been suggested that an extracellular matrix - and cell surface - associated glycoprotein, fibronectin, plays a role in the positioning of cells in morphogenesis and in the maintenance of orderly tissue organization. In the present study the appearance and distribution of fibronectin during in ovo chick limb development has been investigated by(More)