Abdelkader Essafi

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The epicardial epithelial-mesenchymal transition (EMT) is hypothesized to generate cardiovascular progenitor cells that differentiate into various cell types, including coronary smooth muscle and endothelial cells, perivascular and cardiac interstitial fibroblasts and cardiomyocytes. Here we show that an epicardial-specific knockout of the gene encoding(More)
Sonic hedgehog (Shh) expression during limb development is crucial for specifying the identity and number of digits. The spatial pattern of Shh expression is restricted to a region called the zone of polarizing activity (ZPA), and this expression is controlled from a long distance by the cis-regulator ZRS. Here, members of two groups of ETS transcription(More)
Pierre Robin sequence (PRS) is an important subgroup of cleft palate. We report several lines of evidence for the existence of a 17q24 locus underlying PRS, including linkage analysis results, a clustering of translocation breakpoints 1.06-1.23 Mb upstream of SOX9, and microdeletions both approximately 1.5 Mb centromeric and approximately 1.5 Mb telomeric(More)
Wt1 regulates the epithelial-mesenchymal transition (EMT) in the epicardium and the reverse process (MET) in kidney mesenchyme. The mechanisms underlying these reciprocal functions are unknown. Here, we show in both embryos and cultured cells that Wt1 regulates Wnt4 expression dichotomously. In kidney cells, Wt1 recruits Cbp and p300 as coactivators; in(More)
A recent genome-wide association study identified hepatocyte nuclear factor 1-α (HNF1A) as a key regulator of fucosylation. We hypothesized that loss-of-function HNF1A mutations causal for maturity-onset diabetes of the young (MODY) would display altered fucosylation of N-linked glycans on plasma proteins and that glycan biomarkers could improve the(More)
Over half of all proteins are glycosylated, and alterations in glycosylation have been observed in numerous physiological and pathological processes. Attached glycans significantly affect protein function; but, contrary to polypeptides, they are not directly encoded by genes, and the complex processes that regulate their assembly are poorly understood. A(More)
Sonic hedgehog (Shh) expression in the limb bud organizing center called the ZPA is regulated by the ZRS enhancer. Here, we examine in mouse and in a mouse limb-derived cell line the dynamic events that activate and restrict the spatial activity of the ZRS. FGF signalling in the distal limb primes the ZRS at early embryonic stages maintaining a poised, but(More)
could not be detected in previous studies, passed through lamina g and invaded deeper laminae such as i/j in SGFS. These invading fibers appear to degenerate as the development proceeds, suggesting that these are pruned at later stages. Based on these results, we suppose a following mechanism. The retinal axons initially invade not only upper laminae but(More)
tem, where distal lung tissue from E18.5 Glucocorticoid Receptor (GR)–null mice have been exposed to at-RA. Whole genome microarrays and quantitative Real-time PCR have been utilized to identify and confirm GR-antagonised RA-responsive genes. Only the common rarb2/4 transcript and tcf15 (paraxis) were found to match this criteria. We propose that the(More)
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