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Structural investigation of chondroitin/dermatan sulfate oligosaccharides from human skin fibroblast decorin.
In a first attempt to identify the biologically active sequence, a novel protocol for structural analysis of enzyme-resistant oligosaccharide larger than standard trisulfated hexasaccharides was developed. Expand
Continuous Parasacral Sciatic Block: A Radiographic Study
It is concluded that the parasacral sciatic block results in frequent success of blockade of all three major components of the sciatic plexus and it has a small risk of complications. Expand
Danazol therapy in patients with chronic idiopathic thrombocytopenic purpura: long-term results.
The findings suggest that danazol therapy may be beneficial in the management of refractory chronic ITP or when there are contraindications to splenectomy or corticosteroids (or both). Expand
Screening and sequencing of complex sialylated and sulfated glycosphingolipid mixtures by negative ion electrospray Fourier transform ion cyclotron resonance mass spectrometry
A protocol for negative ion nanoelectrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (−)nanoESI-FTICR MS, investigation of complex biological mixtures consisting ofExpand
Infraclavicular Plexus Block: Multiple Injection Versus Single Injection
By performing an infraclavicular block with stimulation of all 3 cords of the brachial plexus, the success rate is higher than when only a single stimulation is used. Expand
Galactosaminoglycan Function and Oligosaccharide Structure Determination
The importance of sequencing long chondroitin sulfate and dermatan sulfate chains specifically derived from decorin, a member of the small leucine-rich repeat proteoglycans and ubiquitously expressed primarily in the skin, is discussed. Expand
Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: substrate specificity, polymerizing properties and usage of different acceptors for fructosylation.
Expectedly, site-directed mutagenesis of His321 in Lsc3 to Arg, Lys, Leu and Ser resulted in proteins with decreased catalytic activity, affinity for sucrose and polymerizing ability, as well as a Lsc2 mutant Thr302Pro with reduced synthesis of levan and long-chain FOS. Expand
Identification of a high-affinity-binding oligosaccharide by (+) nanoelectrospray quadrupole time-of-flight tandem mass spectrometry of a noncovalent enzyme-ligand complex.
A novel method based on nanoESI quadrupole time-of-flight collision-induced dissociation tandem mass spectrometry (nanoESI-QTOFCID MS/MS) was employed for top-down sequencing of the complex-generating fragment ions of the bound ligand, which confirmed the specificity and stoichiometry of enzyme–ligand recognition. Expand
Recent advances in sheathless interfacing of capillary electrophoresis and electrospray ionization mass spectrometry.
  • A. Zamfir
  • Medicine, Chemistry
  • Journal of chromatography. A
  • 3 August 2007
The current concepts and methods for in-laboratory production of sturdy designs based on either conductive emitters or electrodeless interfaces are described and the advantages, limitations and feasibility for certain applications of these systems as well as the perspectives for their performance improvement are concurrently assessed. Expand
Structural characterization of chondroitin/dermatan sulfate oligosaccharides from bovine aorta by capillary electrophoresis and electrospray ionization quadrupole time-of-flight tandem mass
An analytical approach based on high-performance capillary electrophoresis (CE) in conjunction with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-QTOF-MS/MS) hasExpand