• Publications
  • Influence
High-throughput screening for induced point mutations.
TLDR
Adaptations of the targeting induced local lesions in genomes are reported, which help to understand gene function and effective reverse genetic strategies are increasingly in demand. Expand
Mutation detection using a novel plant endonuclease.
TLDR
The results indicate that CEL I mutation detection is a highly sensitive method for detecting both polymorphisms and disease-causing mutations in DNA fragments as long as 1120 bp in length. Expand
MED1, a novel human methyl-CpG-binding endonuclease, interacts with DNA mismatch repair protein MLH1.
TLDR
It is suggested that MED1 is a novel human DNA repair protein that may be involved in MMR and, as such, may be a candidate eukaryotic homologue of the bacterial MMR endonuclease, MutH. Expand
Enhanced cisplatin cytotoxicity by disturbing the nucleotide excision repair pathway in ovarian cancer cell lines.
TLDR
It is suggested that it is possible to substantially enhance the cisPlatin cytotoxicity by disturbing the NER pathway in cisplatin-resistant cell lines and to enhance the survival capacity of mice in an ovarian cancer xenograft model. Expand
Biphasic Kinetics of the Human DNA Repair Protein MED1 (MBD4), a Mismatch-specific DNA N-Glycosylase*
TLDR
It is shown that MED1 functions as a mismatch-specific DNA N-glycosylase active on thymine, uracil, and 5-fluorouracil when these bases are opposite to guanine, and that the 5-methylcytosine binding domain and methylation of the mismatched CpG site are not required for efficient catalysis. Expand
Histone modifications silence the GATA transcription factor genes in ovarian cancer
TLDR
Altered histone modification of the promoter loci is one mechanism responsible for the silencing of GATA transcription factors and the subsequent loss of a target gene, the tumor suppressor Disabled-2, in ovarian carcinogenesis. Expand
Proteomic Analyses of Pancreatic Cyst Fluids
TLDR
Protein nicking by proteases in the cyst fluids produced hundreds of protein spots from the major proteins, making 2-dimensional gel proteomics unmanageable, and a panel of potential biomarker proteins that correlated with carcinoembryonic antigen (CEA) was revealed. Expand
Purification, cloning, and characterization of the CEL I nuclease.
TLDR
It is proposed that CEL I exemplifies a new family of neutral pH optimum, magnesium-stimulated, mismatch duplex-recognizing nucleases, within the S1 superfamily, which includes nucleases putatively encoded by the genes BFN1 of Arabidopsis, ZEN1 of Zinnia, and DSA6 of daylily. Expand
Investigation of the substrate spectrum of the human mismatch‐specific DNA N‐glycosylase MED1 (MBD4): Fundamental role of the catalytic domain
TLDR
It is shown that MED1 works under a wide range of temperature and pH, and has a limited optimum range of ionic strength, and the isolated catalytic domain of MED1 retains the preference for G:T and G:U substrates in the context of methylated or unmethylated CpG sites, suggesting that the catalyticdomain is fundamental and the 5‐methylcytosine binding domain dispensable in determining the substrate spectrum of MED2. Expand
Assessment of two immunodepletion methods: off-target effects and variations in immunodepletion efficiency may confound plasma proteomics.
TLDR
The more profound immunodepletion achieved with the IgY14+SuperMix may lead to false-positive fold-changes between comparison groups if the reproducibility and efficiency of the depletion of a given protein are not considered. Expand
...
1
2
3
4
5
...