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A New Insect-Specific Flavivirus from Northern Australia Suppresses Replication of West Nile Virus and Murray Valley Encephalitis Virus in Co-infected Mosquito Cells
It is demonstrated that prior infection of cultured mosquito cells with PCV suppressed subsequent replication of the medically significant West Nile and Murray Valley encephalitis viruses by 10–43 fold, suggesting that superinfection exclusion can occur between ISFs and vertebrate-infecting flaviviruses despite their high level of genetic diversity. Expand
Production of site-specific antibody-drug conjugates using optimized non-natural amino acids in a cell-free expression system.
- E. Zimmerman, Tyler H. Heibeck, +16 authors A. Sato
- Medicine, Chemistry
- Bioconjugate chemistry
- 29 January 2014
A cell-free protein expression system for production of antibody drug conjugates is established through site-specific incorporation of the optimized non-natural amino acid, para-azidomethyl-l-phenylalanine (pAMF), and a novel variant of the Methanococcus jannaschii tyrosyl tRNA synthetase (TyrRS), with a high activity and specificity toward pAMF is discovered. Expand
ARID5B as a critical downstream target of the TAL1 complex that activates the oncogenic transcriptional program and promotes T-cell leukemogenesis.
The results indicate that ARID5B reinforces the oncogenic transcriptional program by positively regulating the TAL1-induced regulatory circuit and MYC in T-ALL, thereby contributing to T-cell leukemogenesis. Expand
Targeting CD74 in multiple myeloma with the novel, site-specific antibody-drug conjugate STRO-001
Data suggest that STRO-001 is a promising therapeutic agent for the treatment of MM and induced dose-responsive, reversible B-cell and monocyte depletion in cynomolgus monkeys, with no evidence of off-target toxicity. Expand
Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins
A protein additive screen was undertook to identify chaperone proteins that improve the folding and assembly of trastuzumab in OCFS and identified the disulfide isomerase DsbC and the prolyl isomerases FkpA as important positive effectors of IgG folding. Expand
RF1 attenuation enables efficient non-natural amino acid incorporation for production of homogeneous antibody drug conjugates
The genome engineering of a RF1 mutant strain is described that enhances suppression efficiency during cell-free protein synthesis, without significantly impacting cell growth during biomass production, resulting in a scaleable extract production process. Expand
Viral RNA Intermediates as Targets for Detection and Discovery of Novel and Emerging Mosquito-Borne Viruses
- Caitlin A O'Brien, J. Hobson-Peters, +9 authors R. Hall
- Biology, Medicine
- PLoS neglected tropical diseases
- 1 March 2015
Two monoclonal antibodies which show co-localised staining with proteins involved in viral RNA replication in immunofluorescence assay (IFA), suggesting specific reactivity to viral dsRNA are developed and incorporated into a high-throughput, economical ELISA-based screening system for the detection and discovery of viruses from mosquito populations. Expand
Enhancer profiling identifies critical cancer genes and characterizes cell identity in adult T-cell leukemia.
A number of studies have recently demonstrated that super-enhancers, which are large cluster of enhancers typically marked by a high level of acetylation of histone H3 lysine 27 and mediator… Expand
Targeting CD74 with Novel Antibody Drug Conjugates (ADCs) for the Treatment of B-Cell Non-Hodgkin's Lymphoma (NHL)
Novel CD74-targeting ADCs, SP7676 and SP7675 (STRO-001) that exhibit a high degree of homogeneity characterized by the drug linker covalently binding to a single defined site are generated and have potential for improved PK, safety and activity profiles. Expand
Abstract 1782: Discovery and activity of STRO-002, a novel ADC targeting folate receptor alpha for ovarian and endometrial cancer
Folate receptor alpha (FolRα) is a glycosylphosphatidylinositol linked cell-surface glycoprotein that is widely expressed in serous and epithelial ovarian cancer, endometrial adenocarcinoma,… Expand