• Publications
  • Influence
Evolution and classification of the CRISPR–Cas systems
TLDR
An updated analysis of the evolutionary relationships between CRISPR–Cas systems and Cas proteins is provided and a 'polythetic' classification that integrates the phylogenies of the most common cas genes, the sequence and organization of theCRISPR repeats and the architecture of the CRISpr–cas loci is proposed.
An updated evolutionary classification of CRISPR–Cas systems
TLDR
An approach combining the analysis of signature protein families and features of the architecture of cas loci that unambiguously partitions most CRISPR–cas loci into distinct classes, types and subtypes is presented.
Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
TLDR
An updated evolutionary classification of CRISPR–Cas systems and cas genes is provided, with an emphasis on the major developments that have occurred since the publication of the latest classification, in 2015, which includes 2 classes, 6 types and 33 subtypes.
A dual function of the CRISPR–Cas system in bacterial antivirus immunity and DNA repair
TLDR
The results show that YgbT belongs to a novel, structurally distinct family of nucleases acting on branched DNAs and suggest that, in addition to antiviral immunity, at least some components of the CRISPR–Cas system have a function in DNA repair.
Nuclease Activity of the Human SAMHD1 Protein Implicated in the Aicardi-Goutières Syndrome and HIV-1 Restriction*
TLDR
It is shown that the purified full-length human SAMHD1 protein also possesses metal-dependent 3′→5′ exonuclease activity against single-stranded DNAs and RNAs in vitro and could represent an additional mechanism contributing to HIV-1 restriction and suppression of the autoimmune response through direct cleavage of viral and endogenous nucleic acids.
A Novel Family of Sequence-specific Endoribonucleases Associated with the Clustered Regularly Interspaced Short Palindromic Repeats*
TLDR
CAS2 proteins are sequence-specific endoribonucleases, and it is proposed that their role in the CRISPR-mediated anti-phage defense might involve degradation of phage or cellular mRNAs.
Genome-wide Analysis of Substrate Specificities of the Escherichia coli Haloacid Dehalogenase-like Phosphatase Family*
TLDR
Using a representative set of 80 phosphorylated substrates, the substrate specificities of 23 soluble HADs encoded in the Escherichia coli genome are characterized and the physiological relevance of the phosphatase activity with the preferred substrate was validated in vivo for one of the HADS, YniC.
Polyphosphate-dependent synthesis of ATP and ADP by the family-2 polyphosphate kinases in bacteria
TLDR
The PPK2s represent a molecular mechanism, which potentially allow bacteria to use polyP as an intracellular energy reserve for the generation of ATP and survival.
General Enzymatic Screens Identify Three New Nucleotidases in Escherichia coli
TLDR
Three uncharacterized E. coli proteins that belong to different enzyme superfamilies have different sensitivities to inhibition by various nucleoside di- and triphosphates and their varied substrate specificities suggest that these enzymes play unique functions in the intracellular nucleotide metabolism inE.
...
1
2
3
4
5
...