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Robust Growth of Escherichia coli
TLDR
The long-term growth and division patterns of Escherichia coli cells are studied by employing a microfluidic device designed to follow steady-state growth anddivision of a large number of cells at a defined reproductive age to conclude that E. coli has a robust mechanism of growth that is decoupled from cell death. Expand
Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms
TLDR
This analysis suggests that different active processes are involved in movement and localization of the chromosome and of the two plasmids during segregation. Expand
Transcriptional antitermination in the bgl operon of E. coli is modulated by a specific RNA binding protein
TLDR
Evidence is provided that BglG is an RNA binding protein that recognizes a specific sequence located just upstream of each of the terminators, which indicates that the protein requires a specific RNA secondary structure for recognition. Expand
Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes
TLDR
Results indicate that in vivo DNA superhelicity is a result of a balance between topoisomerase I and gyrase activities, and an excess of negative supercoils due to an absence of topoisomersase I is deleterious to the cell, but a moderate gyrases deficiency is not harmful. Expand
Bipolar Localization of the Replication Origin Regions of Chromosomes in Vegetative and Sporulating Cells of B. subtilis
TLDR
The results indicate the existence of a mitotic-like apparatus that is responsible for moving the origin regions of newly formed chromosomes toward opposite ends of the cell. Expand
Autoregulation of the DNA replication gene dnaA in E. coli K-12
TLDR
The results indicate that the dnaA gene product regulates its own synthesis by inhibiting transcription from both of its promoters, and that a site between the two promoters is responsible for the regulation. Expand
High efficiency of HIV-1 genomic RNA packaging and heterozygote formation revealed by single virion analysis
TLDR
This study provides answers to long-standing questions in HIV-1 biology and illustrates the power and sensitivity of the 2-RNA labeling method, which can also be adapted to analyze various issues of RNA biogenesis including the detection of different RNAs in live cell imaging. Expand
Enhancement of bacterial gene expression by insertion elements or by mutation in a CAP-cAMP binding site.
TLDR
The ethylmethane sulfonate-induced point mutations in bglR are alterations in a single base in the cAMP binding protein (CAP) binding site, leading to a stronger binding of the CAP-cAMP complex. Expand
Kinetics of plasmid segregation in Escherichia coli
TLDR
Aberrant positioning of mini‐F lacking its sopC centromere demonstrated the requirement for the partition system in this localization process, and the molecular basis of this clustering, and evidence for phasing of the partition event in the cell cycle are discussed. Expand
Insertion of DNA activates the cryptic bgl operon in E. coli K12
TLDR
Two insertion elements, IS1 and IS5, have been observed to effect activation of the cryptic bgl operon of Escherichia coli K12 and the operon is inducible by βglucosides in a cyclic AMP-dependent manner. Expand
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