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Mitochondrial phosphate transport. Large scale isolation and characterization of the phosphate transport protein from beef heart mitochondria.
Qualitative digestion shows that carboxypeptidase A is able to release at least three amino acids from the C termini of the alpha as well as the beta band of phosphate transport protein. Expand
The proteolytic substructure of light meromyosin. Localization of a region responsible for the low ionic strength insolubility of myosin.
Fragments LF-1, LMM-D, LF-2, and LF-3, with chain masses equal to 63, 56, 47, and 30 kDa, respectively, have been isolated by column chromatography and suggest that a relatively small stretch of peptide located about 100 residues from the COOH terminus of myosin heavy chain is responsible for the insolubility of LMM at low ionic strength. Expand
Glutamic acid-88 is close to SH-1 in the tertiary structure of myosin subfragment 1.
Peptides containing cross-links were isolated by liquid chromatography and subjected to amino acid sequence analyses and show that Glu-88 is the major site and Asp-89 and Met-92 are the minor sites involved in cross-linking with SH-1 (Cys-707) via BPIA. Expand
Engineering Upper Hinge Improves Stability and Effector Function of a Human IgG1
The substitution of His229 with Tyr showed promising advantages over the native antibody and other substitutions in improving the stability and function of the IgG1, and it is proposed that the lower redox potential of Tyr, a residue that may be the ultimate sink for oxidizing equivalents in proteins, is responsible for the inhibition. Expand
The amino acid sequence and stability predictions of the hinge region in myosin subfragment 2.
  • R. Lu, A. Wong
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • 25 March 1985
Comparison of the sequence with that of other portions of the rod, viz. short subfragment 2 and light meromyosin, and of tropomyOSin shows that the hinge region shares some feature of a coiled-coil helical structure, but it has somewhat fewer hydrophobic coil- coil interactions and there is a significant number of charged residues in the Hydrophobic core region. Expand
Both the 25-kDa and 50-kDa domains in myosin subfragment 1 are close to the reactive thiols.
  • R. Lu, L. Moo, A. Wong
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 September 1986
The thiol-specific photoactivatable reagent benzophenone-4-iodoacetamide can be incorporated into myosin subfragment 1 (S1), accompanied by an increase of Ca2+-ATPase and the loss of K+-ATPaseExpand
Evidence Supporting the 19 β-Strand Model for Tom40 from Cysteine Scanning and Protease Site Accessibility Studies*
Data support the 19-strand model for Tom40 with the C-terminal end of the protein localized to the intermembrane space and substituted cysteine accessibility mapping is used to identify several potential β-strands in the Tom40 protein in isolated mitochondria. Expand
Real-time immuno-polymerase chain reaction in a 384-well format: detection of vascular endothelial growth factor and epidermal growth factor-like domain 7.
A 384-well immuno-PCR method that uses streptavidin coated on a PCR plate to capture complexes of biotinylated capture antibody, antigen, and DNA-labeled detection antibody, which can be used to develop high-throughput biomarker assays. Expand
Phosphorylation changes the spatial relationship between Glu124-Arg143 and Cys18 and Cys165 of the regulatory light chain in smooth muscle myosin.
Results suggest that the region of Glu124-Arg143 is involved in the phosphorylation-dependent signaling and the change in its spatial relationship with respect to the N and C termini of RLC may underlie the activation of the smooth muscle myosin. Expand