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Characterization and partial purification of an enantioselective arylacetonitrilase from Pseudomonas fluorescens DSM 7155
Pseudomonas fluorescens DSM 7155 after growth on phenylacetonitrile as sole nitrogen source contained an inducible nitrilase which consists of two different functional subunits (40 and 38 kDa). TheExpand
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Enzymatic Baeyer–Villiger oxidations of some bicyclo[2.2.1]heptan-2-ones using monooxygenases from Pseudomonas putida NCIMB 10007: enantioselective preparation of a precursor of azadirachtin
Two monooxygenases MO1 (NADH dependent) and MO2 (NADPH dependent) isolated from Ps. putida NCIMB 10007 [grown on (+)-camphor] have been utilized as biocatalysts in Baeyer–Villiger oxidations. TheExpand
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Oxidations by microbial NADH plus FMN-dependent luciferases from Photobacterium phosphoreum and Vibrio fischeri
Abstract The NADH plus FMN-dependent luciferase from Photobacterium phosphoreum NCIMB 844 has been shown to act as a Baeyer-Villiger monooxygenase able to perform regio-, and where relevant,Expand
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Ester formation from ethanol by Candida pseudotropicalis
  • A. Willetts
  • Chemistry, Medicine
  • Antonie van Leeuwenhoek
  • 1 August 1989
The production of ethanol, acetate ion and ethyl acetate from glucose by the yeast Candida pseudotropicalis NCYC 143 was investigated under aerobic and anaerobic growth conditions. Acetate and ethylExpand
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Biological Baeyer–Villiger oxidation of some monocyclic and bicyclic ketones using monooxygenases from Acinetobacter calcoaceticus NCIMB 9871 and Pseudomonas putida NCIMB 10007
A. calcoaceticus NCIMB 9871 and Ps. putida NCIMB 10007 [grown on (+)-camphor] have been utilized as biocatalysts in Baeyer–Villiger oxidations. The former microorganism oxidized the racemic ketone 6Expand
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Ab initio calculation of anharmonic constants for a transition state, with application to semiclassical transition state tunneling probabilities
“Good” (i.e. conserved) action variables exist in the vicinity of a saddle point (i.e. transition state) of a potential energy surface in complete analogy to those related to a minimum on theExpand
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Enantioselective degradation of the herbicide mecoprop [2-(2-methyl-4-chlorophenoxy) propionic acid] by mixed and pure bacterial cultures
A consortium of three bacteria was isolated from top soil through their capacity to utilise the chlorinated, aromatic herbicide mecoprop as a single growth substrate. The consortium constituted aExpand
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Purification, crystallisation and preliminary X‐ray analysis of the vanadium‐dependent haloperoxidase from Corallina officinalis
The vanadium‐dependent haloperoxidase from the seaweed Corallina officinalis has been purified to homogeneity and crystallised. The protein is reported to be a hexamer of 12 × 64,000 Da, contains noExpand
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Kinetics of overexpressed transketolase from Escherichia coli JM 107/pQR 700
Abstract Initial rate and product inhibition studies using pH-stat unbuffered biotransformation mixtures at 25°C and pH 7.0 have been performed on overexpressed transketolase (EC from aExpand
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Camphor-grown Pseudomonas putida, a multifunctional biocatalyst for undertaking Baeyer-Villiger monooxygenase-dependent biotransformations
SummaryBoth NADH- and NADPH-dependent Baeyer-Villiger monooxygenase activities with potential uses as biocatalysts for biotransformations are present to different extents throughout the growth ofExpand
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