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Separation of cyclic 3',5'-nucleoside monophosphates from other nucleotides on aluminum oxide columns. Application to the assay of adenyl cyclase and guanyl cyclase.
A method is described for separating 3′,5′-nucleoside monophosphates from other nucleotides on columns of aluminum oxide. It was found that 3′,5′-cyclic nucleotides, nucleosides, purines, and… Expand
Detection of guanyl cyclase in mammalian tissues.
Abstract Guanyl cyclase was assayed by determining the rate of conversion of [ α - 32 P ]GTP to cyclic 3′,5′-[ 32 P]GMP. A two-step chromatographic procedure was developed for isolating the product.… Expand
Separation and purification of cyclic nucleotides by alumina column chromatography.
- A. White
- Chemistry, Medicine
- Methods in enzymology
Publisher Summary Alumina is a useful adsorbent for the anion exchange chromatography of 3', 5'-cyclic nucleotides because it effects a sharp discrimination on the basis of charge. The univalent… Expand
Improved two-step method for the assay of adenylate and guanylate cyclase.
Abstract A two-step assay for adenylate and guanylate cyclase is described utilizing α- 32 P-labeled ATP or GTP as substrate and involving purification of the resulting 32 P-labeled cAMP or cGMP by… Expand
Activation of rat lung soluble guanylate cyclase by sodium nitroprusside: effects of hemoglobin and reducing agents.
Abstract A potent vasodilator, sodium nitroprusside, activated rat lung soluble guanylate cyclase about 2.0-fold; this activation was potentiated by reducing agents such as ascorbic acid and thiols,… Expand
Cytological Detection of Parathyroid Hormone by Immunofluorescence
- G. K. Hargis, V. Yakulis, G. Williams, A. White
- Proceedings of the Society for Experimental…
- 1 December 1964
Summary The fluorescent antibody tech™ nic has been utilized to detect and to localize parathyroid hormone in the chief cells of bovine, human and rat parathyroid tissue. Im-munochemical… Expand
Nitrofurantoin pulmonary toxicity.
An elderly woman taking 50 mg of nitrofurantoin daily for six months for suppression of recurrent urinary tract infections developed increasing dyspnea and bilateral pulmonary infiltrates. An… Expand
Reversal of the biological activity of Escherichia coli heat-stable enterotoxin by disulfide-reducing agents.
- M. Eldeib, C. Dove, C. Parker, T. L. Veum, G. Zinn, A. White
- Biology, Medicine
- Infection and immunity
Various disulfide-reducing agents, mostly thiols and thiol precursors, were examined for their ability to reduce the disulfide bonds in the Escherichia coli heat-stable enterotoxin STa; reduction of… Expand
Nucleotide regulation of heat-stable enterotoxin receptor binding and of guanylate cyclase activation.
Certain nucleotides were found to regulate the binding of the Escherichia coli heat-stable enterotoxin (STa) to its receptor in pig intestinal brush border membranes. ATP and adenine nucleotide… Expand