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Separation of cyclic 3',5'-nucleoside monophosphates from other nucleotides on aluminum oxide columns. Application to the assay of adenyl cyclase and guanyl cyclase.
A method is described for separating 3′,5′-nucleoside monophosphates from other nucleotides on columns of aluminum oxide. It was found that 3′,5′-cyclic nucleotides, nucleosides, purines, andExpand
  • 237
  • 2
Detection of guanyl cyclase in mammalian tissues.
Abstract Guanyl cyclase was assayed by determining the rate of conversion of [ α - 32 P ]GTP to cyclic 3′,5′-[ 32 P]GMP. A two-step chromatographic procedure was developed for isolating the product.Expand
  • 178
  • 1
Separation and purification of cyclic nucleotides by alumina column chromatography.
  • A. White
  • Chemistry, Medicine
  • Methods in enzymology
  • 1974
Publisher Summary Alumina is a useful adsorbent for the anion exchange chromatography of 3', 5'-cyclic nucleotides because it effects a sharp discrimination on the basis of charge. The univalentExpand
  • 90
  • 1
Improved two-step method for the assay of adenylate and guanylate cyclase.
Abstract A two-step assay for adenylate and guanylate cyclase is described utilizing α- 32 P-labeled ATP or GTP as substrate and involving purification of the resulting 32 P-labeled cAMP or cGMP byExpand
  • 77
  • 1
Activation of rat lung soluble guanylate cyclase by sodium nitroprusside: effects of hemoglobin and reducing agents.
Abstract A potent vasodilator, sodium nitroprusside, activated rat lung soluble guanylate cyclase about 2.0-fold; this activation was potentiated by reducing agents such as ascorbic acid and thiols,Expand
  • 12
  • 1
Cytological Detection of Parathyroid Hormone by Immunofluorescence
Summary The fluorescent antibody tech™ nic has been utilized to detect and to localize parathyroid hormone in the chief cells of bovine, human and rat parathyroid tissue. Im-munochemicalExpand
  • 13
  • 1
Nitrofurantoin pulmonary toxicity.
An elderly woman taking 50 mg of nitrofurantoin daily for six months for suppression of recurrent urinary tract infections developed increasing dyspnea and bilateral pulmonary infiltrates. AnExpand
  • 23
  • 1
Reversal of the biological activity of Escherichia coli heat-stable enterotoxin by disulfide-reducing agents.
Various disulfide-reducing agents, mostly thiols and thiol precursors, were examined for their ability to reduce the disulfide bonds in the Escherichia coli heat-stable enterotoxin STa; reduction ofExpand
  • 6
  • 1
Nucleotide regulation of heat-stable enterotoxin receptor binding and of guanylate cyclase activation.
Certain nucleotides were found to regulate the binding of the Escherichia coli heat-stable enterotoxin (STa) to its receptor in pig intestinal brush border membranes. ATP and adenine nucleotideExpand
  • 9
  • 1
Guanylate cyclase activity in heart and lung.
  • A. White
  • Medicine
  • Advances in cyclic nucleotide research
  • 1975
  • 6
  • 1