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New modules for PCR‐based gene targeting in Candida albicans: rapid and efficient gene targeting using 100 bp of flanking homology region
The use of PCR‐based techniques for directed gene alterations has become a standard tool in Saccharomyces cerevisiae. In our efforts to increase the speed of functional analysis of Candida albicansExpand
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Genome Sequence of Saccharomyces carlsbergensis, the World’s First Pure Culture Lager Yeast
Lager yeast beer production was revolutionized by the introduction of pure culture strains. The first established lager yeast strain is known as the bottom fermenting Saccharomyces carlsbergensis,Expand
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An improved transformation protocol for the human fungal pathogen Candida albicans
Abstract. Commonly used protocols for the transformation of the dimorphic human fungal pathogen Candida albicans rely on established methods for the yeast Saccharomyces cerevisiae. With respect toExpand
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Ashbya gossypii: a model for fungal developmental biology
Ashbya gossypii is a riboflavin-overproducing filamentous fungus that is closely related to unicellular yeasts such as Saccharomyces cerevisiae. With its close ties to yeast and the ease of geneticExpand
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Candida albicans CHT3 encodes the functional homolog of the Cts1 chitinase of Saccharomyces cerevisiae.
Chitin synthesis and chitin degradation play an important role in cellular morphogenesis and influence the cell shape of fungal organisms. The Candida albicans genome contains four chitinase genes,Expand
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PCR-based gene targeting in Candida albicans
PCR-based gene-targeting approaches have increased the speed of gene function analyses in ascomycetous fungi, for example, in the diploid human fungal pathogen Candida albicans. Here we describe aExpand
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Yap1-dependent oxidative stress response provides a link to riboflavin production in Ashbya gossypii.
Ashbya gossypii is a natural overproducer of riboflavin. Overproduction of riboflavin can be induced by environmental stress, e.g. nutritional or oxidative stress. The Yap-protein family has aExpand
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New pFA‐cassettes for PCR‐based gene manipulation in Candida albicans
Several modules for efficient PCR‐based gene disruption have recently been introduced in Candida albicans. These are based on auxotrophic marker genes for deficient strains derived from SC5314/CAI4.Expand
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Ras1-Induced Hyphal Development in Candida albicans Requires the Formin Bni1
ABSTRACT Formins are downstream effector proteins of Rho-type GTPases and are involved in the organization of the actin cytoskeleton and actin cable assembly at sites of polarized cell growth. HereExpand
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