Transformation of Pseudomonas aeruginosa by electroporation.
- A. W. Smith, B. Iglewski
- BiologyNucleic Acids Research
- 25 December 1989
A method to reproducibly electroporate plasmid DNA into P. aeruginosa based on a modification of the protocol developed by Dower et al. for E. coli, which has application where transfonnation by chemical methods is inefficient or when plasmids cannot be mobilized by conjugation.
Calicivirus emergence from ocean reservoirs: zoonotic and interspecies movements.
- A. W. Smith, D. Skilling, N. Cherry, J. Mead, D. Matson
- BiologyEmerging Infectious Diseases
- 1998
The history of animal caliciviruses, their eventual recognition as zoonotic agents, and their potential usefulness as a predictive model for noncultivatable human and other animal CALiciviral viruses (e.g., those seen in association with rabbit hemorrhagic disease).
Three-dimensional structure of calicivirus.
- B. Prasad, D. Matson, A. W. Smith
- BiologyJournal of Molecular Biology
- 14 July 1994
The three-dimensional structure of a primate calicivirus is determined using electron cryomicroscopy and computer image-processing techniques and suggests the presence of a hinge region that may facilitate the capsid protein to adapt to the three quasi-equivalent environments of the T = 3 icosahedral structure.
Genomic mapping of a calicivirus VPg
- D. Dunham, X. Jiang, T. Berke, A. W. Smith, D. Matson
- BiologyArchives of Virology
- 1998
A primate calicivirus (Pan-1) VPg was identified in Pan-1-infected cells and proteinase K treatment abolished the infectivity of Pan- 1 RNA, suggesting that Pan-2 VPg is required for RNA infectivity.
Prevalence and Virulence Factors of Escherichia coli Serogroups O26, O103, O111, and O145 Shed by Cattle in Scotland
- M. C. Pearce, J. Evans, J. Low
- Medicine, BiologyApplied and Environmental Microbiology
- 1 January 2006
A national survey was conducted to determine the prevalence of Escherichia coli O26, O103, O111, and O145 in feces of Scottish cattle, and the presence of an animal shedding one of these serogroups was a positive predictor for shedding by others, suggesting local transmission of infection.
Cloning of an Enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci
- A. Lowe, P. Lambert, A. W. Smith
- Biology, MedicineInfection and Immunity
- 1 February 1995
Serum from a patient with Enterococcus faecalis endocarditis was used to identify the gene efaA cloned in Lambda ZapII in Escherichia coli, and it is hypothesize that EfaA may function as an adhesin inendocarditis.
Concentration and Detection of Caliciviruses in Water Samples by Reverse Transcription-PCR
- P. Huang, D. Laborde, V. R. Land, D. Matson, A. W. Smith, X. Jiang
- Environmental ScienceApplied and Environmental Microbiology
- 1 October 2000
A method to concentrate and detect HuCVs in water samples by using a cultivable primate calicivirus (Pan-1) as a model, which was more sensitive to high-pH treatment than poliovirus was.
Siderophore-specific induction of iron uptake in Pseudomonas aeruginosa.
- K. Gensberg, K. Hughes, A. W. Smith
- BiologyJournal of General Microbiology
- 1 November 1992
The results suggest that P. aeruginosa can respond specifically to the presence of siderophore and moreover that not only can the pyoverdin receptor transport its cognate ferri-pyoverdin but also different ferri -pyoverdins, albeit at a reduced rate.
Temporal Shedding Patterns and Virulence Factors of Escherichia coli Serogroups O26, O103, O111, O145, and O157 in a Cohort of Beef Calves and Their Dams
- M. C. Pearce, C. Jenkins, G. Frankel
- Biology, MedicineApplied and Environmental Microbiology
- 1 March 2004
For E. coli O26, O103, and O157, there was no association between shedding by calves and shedding by dams within 1 week of birth, and there wasno significant change in shedding following housing.
Distribution of Escherichia coli O157 in Bovine Fecal Pats and Its Impact on Estimates of the Prevalence of Fecal Shedding
- M. C. Pearce, D. Fenlon, G. Gunn
- Medicine, BiologyApplied and Environmental Microbiology
- 1 October 2004
It is probable that most surveys have greatly underestimated the prevalence of E. coli O157 shedding in cattle and the proportion of farms with shedding cattle.
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