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Why barcode? High-throughput multiplex sequencing of mitochondrial genomes for molecular systematics
TLDR
The 454/Roche platform procedure could be employed routinely for mitochondrial genome sequencing at the species level, to provide improved species ‘barcodes’ that currently use the cox1 gene only.
Genetic criteria for establishing evolutionarily significant units in Cryan's buckmoth
TLDR
Investigations showed that the Cryan's buckmoths clearly belong to the Hemileuca maia species group, but they could not be readily distinguished from other members of that group by means of molecular genetic techniques, suggesting recent divergence.
Incongruence of mitochondrial and nuclear gene trees in the Carabid beetles Ohomopterus.
TLDR
The mtDNA gene tree obtained from 44 individuals representing all 15 currently recognized species of Ohomopterus revealed that haplotypes isolated from individuals of a single "species" were frequently separated into distant clades, confirming the previous report.
How slippage-derived sequences are incorporated into rRNA variable-region secondary structure: implications for phylogeny reconstruction.
TLDR
The secondary structure in the cicindelid V4 region may be conserved due to the dynamics of the mutational mechanism rather than to functional constraints, and these processes may also have a tendency to produce similar primary sequences irrespective of phylogenetic associations.
Multiple ancient origins of neoteny in Lycidae (Coleoptera): consequences for ecology and macroevolution
TLDR
A phylogenetic analysis of Lycidae based on DNA sequences from nuclear (18S and 28S rRNA) and mitochondrial (rrnL, cox1, cob and nad5) genes from a representative set of lineages supported three independent origins of neotenic taxa, contradicting earlier suggestions of recent evolution from dispersive non-neotenics.
Extensive trans‐species mitochondrial polymorphisms in the carabid beetles Carabus subgenus Ohomopterus caused by repeated introgressive hybridization
TLDR
The results demonstrate that introgressive hybridization has occurred multiple times, at least for mitochondria, despite differences among, and stability within, morphological characters that distinguish local populations, and could have been key processes in the evolutionary diversification of Ohomopterus.
Rapid RT-PCR-based protein truncation test in the screening for 5' located mutations of the APC gene.
TLDR
An alternative hotspot for non-sense mutations associated with CHRPE appears to encompass the codons 215, 216 and 232, which is, to the authors' knowledge, the fourth exception to the rule that FAP patients manifesting CHRPE harbour genetic alternations downstream from APC exon 9.
Phylogeny of North American Cicindela tiger beetles inferred from multiple mitochondrial DNA sequences.
TLDR
A phylogeny based on 1896 nucleotide positions from three mitochondrial genes (Cytochrome b, Cytochrome oxidase III, and 16S rRNA) yielded widely different tree topologies, but character conflict between them appears to be relatively low.
The discovery of Iberobaeniidae (Coleoptera: Elateroidea): a new family of beetles from Spain, with immatures detected by environmental DNA sequencing
TLDR
Molecular dating shows that Iberobaeniidae represents an ancient relict lineage originating in the Lower Jurassic, which possibly indicates a long history of neoteny, usually considered to be evolutionarily short-lived.
Modelling the secondary structures of slippage-prone hypervariable RNA regions: the example of the tiger beetle 18S rRNA variable region V4.
TLDR
Variable region V4 from cicindelid (tiger beetle) small subunit ribosomal RNAs is model by comparing the results of the MFOLD suboptimal folding algorithm for different species to identify conserved structures and contains a novel structure in the central, highly variable part.
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