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Phosducin‐like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins
TLDR
A role for phosducin‐like proteins in facilitating folding, localization or function of proteins, in addition to modulating G‐protein signalling is established.
Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 degrees C were measured independently by nine laboratories using single-photon timing and multifrequency
Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches
TLDR
Very low cAMP concentrations induce self-organizing PHCrac-GFP patches that serve as a spatial cue for pseudopod formation, which enhances the sensitivity and amplitude of chemotactic movement.
Effects of non-covalent interactions with 5-O-caffeoylquinic acid (chlorogenic acid) on the heat denaturation and solubility of globular proteins.
TLDR
The results indicate that the non-covalent interactions with 5-CQA do not have pronounced effects on the functional properties of globular proteins in food systems.
Structural Dynamics of Green Fluorescent Protein Alone and Fused with a Single Chain Fv Protein*
TLDR
The dynamic properties of GFP alone and fused to a single chain antibody raised against lipopolysaccharide of the outer cell wall of Gram-negative bacteria were investigated and the scFv moiety was functional as was proven in binding assays.
Effects of Refractive Index and Viscosity on Fluorescence and Anisotropy Decays of Enhanced Cyan and Yellow Fluorescent Proteins
TLDR
Time-resolved fluorescence measurements of the enhanced forms of ECFP and EYFP in water–glycerol mixtures were performed to quantify the effects of the refractive index and viscosity on the fluorescence lifetimes of these proteins.
RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium
TLDR
It is concluded that cGMP activates GbpC, mediating the chemoattractant-induced establishment of cell polarity through myosin, resulting in increased attachment and suppression of polarity.
Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization.
TLDR
It is shown that photon counting histogram analysis and dual-color fluorescence cross correlation spectroscopy are able to monitor fluorescently labeled proteins at the single-molecule detection level in living plant cells.
Fluorescence correlation spectroscopy of GFP fusion proteins in living plant cells.
TLDR
This chapter presents an overview of the fluorescence correlation spectroscopy (FCS) of green fluorescent protein (GFP) fusion proteins in living plant cells, including enzyme kinetics, nucleotide hybridization, conformational dynamics in DNA, and protein-DNA interactions.
Sensitive Spectroscopic Detection of Large and Denatured Protein Aggregates in Solution by Use of the Fluorescent Dye Nile Red
TLDR
The results with β-galactosidase demonstrate the potential of Nile red for developing complementary analytical methods that overcome the size limitations of SEC, and can detect the formation of large protein aggregates at early stages.
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