Phosducin‐like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins
A role for phosducin‐like proteins in facilitating folding, localization or function of proteins, in addition to modulating G‐protein signalling is established.
Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 degrees C were measured independently by nine laboratories using single-photon timing and multifrequency…
Structural Dynamics of Green Fluorescent Protein Alone and Fused with a Single Chain Fv Protein*
The dynamic properties of GFP alone and fused to a single chain antibody raised against lipopolysaccharide of the outer cell wall of Gram-negative bacteria were investigated and the scFv moiety was functional as was proven in binding assays.
Effects of non-covalent interactions with 5-O-caffeoylquinic acid (chlorogenic acid) on the heat denaturation and solubility of globular proteins.
- S. Prigent, H. Gruppen, A. Visser, G. V. van Koningsveld, G. D. de Jong, A. Voragen
- Chemistry, BiologyJournal of Agricultural and Food Chemistry
- 12 July 2003
The results indicate that the non-covalent interactions with 5-CQA do not have pronounced effects on the functional properties of globular proteins in food systems.
Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches
- M. Postma, J. Roelofs, J. Goedhart, H. Loovers, A. Visser, P. V. van Haastert
- BiologyJournal of Cell Science
- 15 June 2004
Very low cAMP concentrations induce self-organizing PHCrac-GFP patches that serve as a spatial cue for pseudopod formation, which enhances the sensitivity and amplitude of chemotactic movement.
Effects of Refractive Index and Viscosity on Fluorescence and Anisotropy Decays of Enhanced Cyan and Yellow Fluorescent Proteins
Time-resolved fluorescence measurements of the enhanced forms of ECFP and EYFP in water–glycerol mixtures were performed to quantify the effects of the refractive index and viscosity on the fluorescence lifetimes of these proteins.
Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization.
It is shown that photon counting histogram analysis and dual-color fluorescence cross correlation spectroscopy are able to monitor fluorescently labeled proteins at the single-molecule detection level in living plant cells.
RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium
It is concluded that cGMP activates GbpC, mediating the chemoattractant-induced establishment of cell polarity through myosin, resulting in increased attachment and suppression of polarity.
Imaging protein-protein interactions in living cells
Both FRET- and FCS-based approaches to study protein-protein interactions in vivo are reviewed, combining the high spatial resolution of microscopy with spectroscopic techniques to obtain information about the dynamical behaviour of molecules.