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Thiazolidinone CFTR inhibitor identified by high-throughput screening blocks cholera toxin-induced intestinal fluid secretion.
Secretory diarrhea is the leading cause of infant death in developing countries and a major cause of morbidity in adults. The cystic fibrosis transmembrane conductance regulator (CFTR) protein isExpand
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Green fluorescent protein as a noninvasive intracellular pH indicator.
It was found that the absorbance and fluorescence of green fluorescent protein (GFP) mutants are strongly pH dependent in aqueous solutions and intracellular compartments in living cells. pHExpand
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TMEM16A Inhibitors Reveal TMEM16A as a Minor Component of Calcium-activated Chloride Channel Conductance in Airway and Intestinal Epithelial Cells*
TMEM16A (ANO1) functions as a calcium-activated chloride channel (CaCC). We developed pharmacological tools to investigate the contribution of TMEM16A to CaCC conductance in human airway andExpand
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Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational
The green fluorescent protein (GFP) was used as a noninvasive probe to quantify the rheological properties of cell cytoplasm. GFP mutant S65T was purified from recombinant bacteria for solutionExpand
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Water and Glycerol Permeabilities of Aquaporins 1–5 and MIP Determined Quantitatively by Expression of Epitope-tagged Constructs inXenopus Oocytes*
The goal of this study was to compare single channel water and glycerol permeabilities of mammalian aquaporins (AQP) 1–5 and the major intrinsic protein of lens fiber (MIP). Each of the six clonedExpand
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Small-molecule correctors of defective DeltaF508-CFTR cellular processing identified by high-throughput screening.
The most common cause of cystic fibrosis (CF) is deletion of phenylalanine 508 (DeltaF508) in the CF transmembrane conductance regulator (CFTR) chloride channel. The DeltaF508 mutation producesExpand
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Green fluorescent protein‐based halide indicators with improved chloride and iodide affinities
The green fluorescent protein YFP‐H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK a. However, a limitation of YFP‐H148Q is its low halide sensitivity, with KExpand
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Aquaporin-4 and brain edema
Aquaporin-4 (AQP4) is a water-channel protein expressed strongly in the brain, predominantly in astrocyte foot processes at the borders between the brain parenchyma and major fluid compartments,Expand
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Diffusion of green fluorescent protein in the aqueous-phase lumen of endoplasmic reticulum.
The endoplasmic reticulum (ER) is the major compartment for the processing and quality control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a noninvasive probe toExpand
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Mechanism-based corrector combination restores ΔF508-CFTR folding and function.
The most common cystic fibrosis mutation, ΔF508 in nucleotide binding domain 1 (NBD1), impairs cystic fibrosis transmembrane conductance regulator (CFTR)-coupled domain folding, plasma membraneExpand
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