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IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel
TLDR
It is shown that NMO-IgG binds selectively to the aquaporin-4 water channel, a component of the dystroglycan protein complex located in astrocytic foot processes at the blood-brain barrier, which may represent the first example of a novel class of autoimmune channelopathy. Expand
Thiazolidinone CFTR inhibitor identified by high-throughput screening blocks cholera toxin-induced intestinal fluid secretion.
TLDR
The most potent compound discovered by screening of structural analogs, CFTR(inh)-172, reversibly inhibited CFTR short-circuit current in less than 2 minutes in a voltage-independent manner with K(I) approximately 300 nM. Expand
Aquaporin-4 deletion in mice reduces brain edema after acute water intoxication and ischemic stroke
TLDR
It is shown that mice deficient in aquaporin-4 (AQP4), a glial membrane water channel, have much better survival than wild-type mice in a model of brain edema caused by acute water intoxication, and suggested that AQP4 inhibition may provide a new therapeutic option for reducingbrain edema in a wide variety of cerebral disorders. Expand
Green fluorescent protein as a noninvasive intracellular pH indicator.
TLDR
The results establish the application of GFP as a targetable, noninvasive indicator of intracellular pH and suggest that GFP pH sensitivity involves simple protonation events at a pH of >5, but both protonations and conformational changes at lower pH. Expand
TMEM16A Inhibitors Reveal TMEM16A as a Minor Component of Calcium-activated Chloride Channel Conductance in Airway and Intestinal Epithelial Cells*
TLDR
It is concluded that TMEM16A carries nearly all CaCCCurrent current in salivary gland epithelium, but is a minor contributor to total CaCC current in airway and intestinal epithelia, and potential development candidates for drug therapy of hypertension, pain, diarrhea, and excessive mucus production. Expand
Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational
TLDR
Measurements of GFP translation and rotation in aqueous dextran solutions support the view that the primary barrier to GFP diffusion is collisional interactions between GFP and macromolecular solutes. Expand
Discovery of Glycine Hydrazide Pore-occluding CFTR Inhibitors
TLDR
Compared with the thiazolidinone CFTR inhibitor CFTRinh-172, GlyH-101 has substantially greater water solubility and rapidity of action, and a novel inhibition mechanism involving occlusion near the external pore entrance, and may be useful as probes of CFTR pore structure, in creating animal models of CF, and as antidiarrheals in enterotoxic-mediated secretory diarrheas. Expand
Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption
TLDR
A fundamental role of water channels in cell migration, which is central to diverse biological phenomena including angiogenesis, wound healing, tumour spread and organ regeneration, is supported. Expand
Green fluorescent protein‐based halide indicators with improved chloride and iodide affinities
TLDR
Improved anion sensitivities with K d down to 2 mM for I−, 40 mM for Cl−, and 10 mM for NO3 − were revealed and their utility in cells was demonstrated using transfected cells expressing the cystic fibrosis transmembrane conductance regulator chloride channel. Expand
Mechanism-based corrector combination restores ΔF508-CFTR folding and function
TLDR
The molecular targets of available correctors are elucidated: class I stabilizes the NBD1-MSD1 and N BD1- MSD2 interfaces, and class II targets NBD2, which stabilizes human ΔF508-NBD1. Expand
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