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VirB/D4-dependent protein translocation from Agrobacterium into plant cells.
TLDR
Fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells showed a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA. Expand
Positive charge is an important feature of the C-terminal transport signal of the VirB/D4-translocated proteins of Agrobacterium.
TLDR
Based on sequence alignments and mutational analysis of VirF, it is concluded that the C-terminal transport signal for recruitment and translocation of effector proteins by the A. tumefaciens VirB/D4 system is hydrophilic and has a net positive charge with a consensus motif of R-X(7)-R-X-R- X-R(n)>. Expand
A bipartite signal mediates the transfer of type IV secretion substrates of Bartonella henselae into human cells.
TLDR
It is exemplarily shown that the C terminus of such a conjugative relaxase mediates protein transfer through the Bartonella henselae VirB/VirD4 system into HEC, which may represent the evolutionary origin of the here defined T4S signal for protein transfer into human cells. Expand
Symbiotic phenotypes and translocated effector proteins of the Mesorhizobium loti strain R7A VirB/D4 type IV secretion system
TLDR
The heterologous translocation of rhizobial proteins by the Agrobacterium VirB/D4 T4SS is the first demonstration that rhzobial effector proteins are translocated into plant cells and confirms functional conservation between the M. loti and A. tumefaciens T4 SS strains. Expand
Exploitation of Eukaryotic Ubiquitin Signaling Pathways by Effectors Translocated by Bacterial Type III and Type IV Secretion Systems
TLDR
This work discusses how bacteria exploit this system to control the timing of their effectors' action by programming them for degradation, to block specific intermediates in mammalian or plant innate immunity, or to target host proteins for degradation by mimicking specific ubiquitin/proteasome system components. Expand
Analysis of Vir protein translocation from Agrobacterium tumefaciens using Saccharomyces cerevisiae as a model: evidence for transport of a novel effector protein VirE3.
TLDR
A novel method to also detect trans-kingdom Vir protein transfer from Agrobacterium to yeast, using the Cre/lox system is described and it is shown that VirE2 and VirF protein transfer was inhibited by the presence of the Osa protein. Expand
Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase.
TLDR
The results presented here clearly demonstrate the feasibility of the Agrobacterium -mediated transformation system, which is generally used for transformation of plants, to obtain site-specific integration in Arabidopsis thaliana. Expand
Recognition of the Agrobacterium tumefaciens VirE2 Translocation Signal by the VirB/D4 Transport System Does Not Require VirE11
TLDR
The results unambiguously show that the VirE1 chaperone is not essential for the recognition of the Vir E2 transport signal by the transport system and the subsequent translocation across the bacterial envelope into host cells. Expand
Stable Recombinase-Mediated Cassette Exchange in Arabidopsis Using Agrobacterium tumefaciens1
TLDR
This work presents a Cre-based targeting strategy in Arabidopsis using recombinase-mediated cassette exchange (RMCE) of transferred DNA (T-DNA) delivered by Agrobacterium tumefaciens, and enables simple and efficient selection of targeting events using the advantages of Agrobacteria-mediated transformation. Expand
Exposing the third chromosome of Burkholderia cepacia complex strains as a virulence plasmid
TLDR
It is shown that c3 is not an essential chromosomal element, rather a large plasmid that encodes virulence, secondary metabolism and other accessory functions in Bcc bacteria. Expand
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