Electroporation of cells in microfluidic devices: a review
- M. Fox, D. C. Esveld, R. Boom
- BiologyAnalytical and Bioanalytical Chemistry
- 14 March 2006
Single-cell content analysis is expected to add further value to the concept of the microfluidic chip and if advanced pulse schemes are employed, such microdevices can also enhance research into intracellular electroporation.
Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity.
- A. Valero, T. Braschler, Alexander Rauch, N. Demierre, Y. Barral, P. Renaud
- BiologyLab on a Chip
- 21 May 2011
A non-invasive and label-free continuous cell sorting technique to analyze and synchronize yeast cell division and maximize sensitivity to the characteristic shape and internal structure changes occurring during the yeast cell cycle, allowing to synchronize the culture in late anaphase.
Optimization of microfluidic single cell trapping for long-term on-chip culture.
This work presents a microfluidic chip with enhanced single cell trapping and on-chip culture performance, and demonstrates the automated separation of the two daughter cells generated upon single cell division.
Micropatterning neural cell cultures in 3D with a multi-layered scaffold.
- A. Kunze, M. Giugliano, A. Valero, P. Renaud
- BiologyBiomaterials
- 1 March 2011
Single cell electroporation on chip
- A. Valero
- Biology
- 12 October 2006
The performance of two types of Lab-on-a-Chip trapping devices was tested using beads and cells, whereas the functionality for single cell electroporation of these chips was verified by means of gene transfection studies.
Link between alginate reaction front propagation and general reaction diffusion theory.
- T. Braschler, A. Valero, L. Colella, K. Pataky, J. Brugger, P. Renaud
- Computer ScienceAnalytical Chemistry
- 25 February 2011
An analytical solution is obtained that the fundamental properties of the alginate reaction front are governed by a single dimensionless parameter λ, and it is shown that the λ parameter is of general importance beyond theAlginate model system, as it can be used to classify known solutions for second-order reaction diffusion schemes, along with the novel solution presented here.
A unified approach to dielectric single cell analysis: impedance and dielectrophoretic force spectroscopy.
- A. Valero, T. Braschler, P. Renaud
- BiologyLab on a Chip
- 7 September 2010
A unified approach for single cell dielectric spectroscopy is presented, current microtechnologies applied in electrical analysis of single cells are discussed based on their closely related physical principles and examples of microfluidic devices are presented.
Gene transfer and protein dynamics in stem cells using single cell electroporation in a microfluidic device.
- A. Valero, J. Post, J. V. van Nieuwkasteele, P. T. ter Braak, W. Kruijer, A. van den Berg
- BiologyLab on a Chip
- 2008
It is predicted that microfluidic devices can be used for highly efficient small-scale 'genetic modification' of cells, and biological experimentation, offering possibilities to study cellular processes at the single cell level.
Apoptotic cell death dynamics of HL60 cells studied using a microfluidic cell trap device.
- A. Valero, F. Merino, A. van den Berg
- BiologyLab on a Chip
- 23 December 2005
The design, fabrication and first results of a microfluidic cell trap device for analysis of apoptosis, a first step towards an integrated apoptosis chip for high-throughput drug screening on a single cellular level are presented.
A miniaturized continuous dielectrophoretic cell sorter and its applications.
- A. Valero, T. Braschler, N. Demierre, P. Renaud
- BiologyBiomicrofluidics
- 29 June 2010
A cell sorting device based on the opposition of dielectrophoretic forces that discriminates between cell types according to their dielectric properties, such as the membrane permittivity and the cytoplasm conductivity that succeeded in sorting viable from nonviable yeast with nearly 100% purity.
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