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UBE2S drives elongation of K11-linked ubiquitin chains by the Anaphase-Promoting Complex
TLDR
It is proposed that Ube2S is a critical, unique component of the APC ubiquitination pathway and has an intrinsic preference for the K11 linkage of ubiquitin that is essential for substrate degradation. Expand
Cell Growth and Size Homeostasis in Proliferating Animal Cells
TLDR
It is concluded that proliferating mammalian cells have an intrinsic mechanism that maintains cell size, and growth rate is size-dependent throughout the cell cycle. Expand
Direct observation of mammalian cell growth and size regulation
TLDR
A microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations is introduced and a decrease in the growth rate variability at the G1-S phase transition is revealed, which suggests the presence of a growth rate threshold for maintaining size homeostasis. Expand
Using buoyant mass to measure the growth of single cells
TLDR
A suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control was used to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells, finding that heavier cells grew faster than lighter cells. Expand
Optimizing Optical Flow Cytometry for Cell Volume-Based Sorting and Analysis
TLDR
This work investigated several different light scattering and fluorescence measurements in several different cell lines, sorting cell fractions from the high and low end of distributions, and measuring volume electronically to determine which sorting strategy yielded the best separated volume distributions. Expand
Size homeostasis in adherent cells studied by synthetic phase microscopy
TLDR
A unique optical method is proposed that can measure the dry mass of thick live cells as accurately as that for thin cells with high computational efficiency and quantify, with unprecedented accuracy, the asymmetry of division in lymphoblasts and epithelial cells. Expand
Co‐regulation proteomics reveals substrates and mechanisms of APC/C‐dependent degradation
TLDR
The regulation of KIFC1 (de‐)phosphorylation and degradation provides insights into the fidelity and proper ordering of substrate degradation by the APC/C during mitosis. Expand
Temporal fluxomics reveals oscillations in TCA cycle flux throughout the mammalian cell cycle
TLDR
A temporal‐fluxomics approach is developed to derive a comprehensive and quantitative view of alterations in metabolic fluxes throughout the mammalian cell cycle by combining pulse‐chase LC‐MS‐based isotope tracing in synchronized cell populations with computational deconvolution and metabolic flux modeling. Expand
Unbiased transcriptome signature of in vivo cell proliferation reveals pro- and antiproliferative gene networks
TLDR
It is demonstrated that a simple binary classification of gene expression in proliferating vs. resting B cells can identify, with remarkable selectivity, global in vivo regulators of the mammalian cell cycle, many of which are also post-translationally regulated by the APC/C E3 ligase. Expand
Gas2l3, a Novel Constriction Site-Associated Protein Whose Regulation Is Mediated by the APC/CCdh1 Complex
TLDR
Overexpression of Gas2l3 specifically interferes with cell abscission, which is the final stage of cell division, when the cutting of the intercellular bridge at the constriction sites occurs, and is suggested to be part of the cellular mechanism that terminates cell division. Expand
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