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Yeast/E. coli shuttle vectors with multiple unique restriction sites.
Two yeast/E. coli shuttle vectors have been constructed. The two vectors, YEp351 and YEp352, have the following properties: (1) they can replicate autonomously in Saccharomyces cerevisiae and in E.… Expand
Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions.
We report yeast/Escherichia coli shuttle vectors suitable for fusing yeast promoter and coding sequences to the lacZ gene of E. coli. The vectors contain a region of multiple unique restriction sites… Expand
SDHA is a tumor suppressor gene causing paraganglioma.
- N. Burnichon, Jean-Jacques Brière, +11 authors A. Gimenez-Roqueplo
- Medicine, Biology
- Human molecular genetics
- 1 August 2010
Mitochondrial succinate-coenzyme Q reductase (complex II) consists of four subunits, SDHA, SDHB, SDHC and SDHD. Heterozygous germline mutations in SDHB, SDHC, SDHD and SDHAF2 [encoding for succinate… Expand
Mss51p and Cox14p jointly regulate mitochondrial Cox1p expression in Saccharomyces cerevisiae
Mutations in SURF1, the human homologue of yeast SHY1, are responsible for Leigh's syndrome, a neuropathy associated with cytochrome oxidase (COX) deficiency. Previous studies of the yeast model of… Expand
Characterization of COX17, a Yeast Gene Involved in Copper Metabolism and Assembly of Cytochrome Oxidase*
Mutations in the COX17 gene of Saccharomyces cerevisiae cause a respiratory deficiency due to a block in the production of a functional cytochrome oxidase complex. Because cox17 mutants are able to… Expand
SCO1 and SCO2 Act as High Copy Suppressors of a Mitochondrial Copper Recruitment Defect in Saccharomyces cerevisiae*
- D. Glerum, A. Shtanko, A. Tzagoloff
- Biology, Medicine
- The Journal of Biological Chemistry
- 23 August 1996
C129/U1 is a respiratory defective mutant of Saccharomyces cerevisiae arrested in cytochrome oxidase assembly due to a mutation in COX17, a nuclear gene encoding a low molecular weight cytoplasmic… Expand
 Cytochrome oxidase from beef heart mitochondria
Publisher Summary This chapter discusses the cytochrome oxidase from beef heart mitochondria. Cytochrome c oxidase is mostly assayed by the spectrophotometric method. The rate of oxidation of… Expand
Shy1p is necessary for full expression of mitochondrial COX1 in the yeast model of Leigh's syndrome
SHY1 codes for a mitochondrial protein required for full expression of cytochrome oxidase (COX) in Saccharomyces cerevisiae. Mutations in the homologous human gene (SURF1) have been reported to cause… Expand
Membrane protein degradation by AAA proteases in mitochondria: extraction of substrates from either membrane surface.
- K. Leonhard, B. Guiard, G. Pellecchia, A. Tzagoloff, W. Neupert, T. Langer
- Biology, Medicine
- Molecular cell
- 1 April 2000
Two AAA proteases, each with its catalytic site at the opposite membrane surface, mediate the ATP-dependent degradation of mitochondrial inner membrane proteins. We demonstrate here that a model… Expand
Involvement of mitochondrial ferredoxin and Cox15p in hydroxylation of heme O
Cox15p is essential for the biogenesis of cytochrome oxidase [Glerum et al., J. Biol. Chem. 272 (1997) 19088–19094]. We show here that cox15 mutants are blocked in heme A but not heme O biosynthesis.… Expand