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Genome sequencing in microfabricated high-density picolitre reactors
TLDR
A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.
Nomenclature of Major Antimicrobial-Resistant Clones of Streptococcus pneumoniae Defined by the Pneumococcal Molecular Epidemiology Network
TLDR
The nomenclature for 16 pneumococcal clones that have contributed to the increase in antimicrobial resistance worldwide and can serve as a prototype for the collaboration of scientists in identifying clones of important human pathogens and as a model for the development of other networks.
An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci
TLDR
The observations suggest that cell-wall synthesis in the presence of β-lactam antibiotics requires the cooperative functioning of the transglycosylasedomain of the native staphylococcal PBP2 and the transpeptidase domain of the P BP2A, a protein imported by S. aureus from an extra species source.
Rapid Pneumococcal Evolution in Response to Clinical Interventions
TLDR
How genomic plasticity within lineages of recombinogenic bacteria can permit adaptation to clinical interventions over remarkably short time scales is detailed.
Tracking the in vivo evolution of multidrug resistance in Staphylococcus aureus by whole-genome sequencing
TLDR
The power of whole-genome sequencing is applied to identify steps in the evolution of multidrug resistance in isogenic S. aureus isolates recovered periodically from the bloodstream of a patient undergoing chemotherapy with vancomycin and other antibiotics.
The evolution of pandemic clones of methicillin-resistant Staphylococcus aureus: identification of two ancestral genetic backgrounds and the associated mec elements.
TLDR
Tentative identification of an evolutionary pathway for the emergence of pandemic MRSA clones is allowed and evidence for the multiple, yet restricted, numbers of acquisition of the mec element by S. aureus is provided.
A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterization.
TLDR
The Tn551 insertion site of the autolysis-deficient Staphylococcus aureus mutant RUSAL2 was cloned and used to identify the autologic gene atl in the parent strain, RN450, which is a bifunctional protein that has an amidase domain and an endo-beta-N-acetylglucosaminidase domain.
Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus
TLDR
A high-molecular-weight PBP (PBP-2a; approximate size, 78,000 daltons) was detected that was only present in the resistant bacteria but not in the isogenic sensitive strain, and in cultures grown at pH 5.2, the extra PBP was not detectable.
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