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P25 and P28 proteins of the malaria ookinete surface have multiple and partially redundant functions
It is shown that P25 and P28 share multiple functions during ookinete/oocyst development and the fact that Sko parasites are efficiently transmitted by the mosquito is a compelling reason for including both target antigens in transmission‐blocking vaccines. Expand
Complementary antioxidant defense by cytoplasmic and mitochondrial peroxiredoxins in Leishmania infantum.
Evidence is shown that both enzymes are active as peroxidases in vivo and that they have complementary roles in parasite protection against oxidative stress. Expand
An approach to functional complementation by introduction of large DNA fragments into Trypanosoma cruzi and Leishmania donovani using a cosmid shuttle vector.
A cosmid shuttle vector which facilitates the introduction of large DNA fragments into Trypanosoma cruzi and Leishmania donovani is constructed and the observation that cosmid DNA can be rescued unaltered from transformed parasites is suggested. Expand
Two linked genes of Leishmania infantum encode tryparedoxins localised to cytosol and mitochondrion.
It is suggested that LiTXN1 and Li TXN2 are part of two distinct antioxidant machineries, one cytosolic, the other mitochondrial, that complement each other to ensure effective defence from several sources of oxidants throughout the development of L. infantum. Expand
Mitochondrial peroxiredoxin functions as crucial chaperone reservoir in Leishmania infantum
It is demonstrated that in the vector-borne protozoan parasite Leishmania infantum, mitochondrial peroxiredoxin (Prx) exerts intrinsic ATP-independent chaperone activity, protecting a wide variety of different proteins against heat stress-mediated unfolding in vitro and in vivo. Expand
Heme as a source of iron to Leishmania infantum amastigotes.
Evidence is presented that a ligand at the surface of amastigotes binds hemin with high-affinity (Kd=0.044nM) and may function in intracellular transport of heme while hemoglobin internalization occurs through a different molecule. Expand
Leishmania Mitochondrial Peroxiredoxin Plays a Crucial Peroxidase-Unrelated Role during Infection: Insight into Its Novel Chaperone Activity
It is shown, for the first time, that these 2-Cys peroxiredoxins can be determinant for pathogenicity independently of their peroxidase activity, and the findings reported here change the paradigm which regards all trypanosomatid 2-cysteine peroxires as peroxide-eliminating devices. Expand
Identification of antibodies to Leishmania silent information regulatory 2 (SIR2) protein homologue during canine natural infections: pathological implications.
The determination of immunoglobulin (Ig) isotypes revealed high levels of total IgG in both symptomatic and asymptomatic animals when compared to IgM, and the IgG2 appeared to be the predominant subclass of Ig present in the sera of infected animals particularly in the case of symptomatic dogs. Expand
Mitochondrial Redox Metabolism in Trypanosomatids Is Independent of Tryparedoxin Activity
The separation of trypanosomatid TXN sequences into two classes is proposed and supported by phylogenetic analysis: i) class I, encoding active TXNs, and ii) class II, coding for TA proteins unlikely to function as TXNs. Expand
The mitochondrial complex I of trypanosomatids - an overview of current knowledge
  • M. Duarte, A. Tomás
  • Biology, Medicine
  • Journal of Bioenergetics and Biomembranes
  • 25 June 2014
Bioinformatic and proteomic analyses allowed the identification of many conserved and trypanosomatid-specific subunits of NADH:ubiquinone oxidoreductase, revealing a multifunctional enzyme capable of performing bioenergetic activities and possibly, also of functioning in fatty acid metabolism. Expand