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Nuclease Activity of the Human SAMHD1 Protein Implicated in the Aicardi-Goutières Syndrome and HIV-1 Restriction*
Background: The human SAMHD1 protein has dNTP triphosphatase activity and is involved in HIV-1 restriction and autoimmune syndrome. Results: Purified SAMHD1 exhibits nuclease activity againstExpand
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Structural insight into the mechanism of c-di-GMP hydrolysis by EAL domain phosphodiesterases.
Cyclic diguanylate (or bis-(3'-5') cyclic dimeric guanosine monophosphate; c-di-GMP) is a ubiquitous second messenger that regulates diverse cellular functions, including motility, biofilm formation,Expand
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Biochemical Diversity of Carboxyl Esterases and Lipases from Lake Arreo (Spain): a Metagenomic Approach
ABSTRACT The esterases and lipases from the α/β hydrolase superfamily exhibit an enormous sequence diversity, fold plasticity, and activities. Here, we present the comprehensive sequence andExpand
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The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes
Most of the Earth’s biosphere is cold and is populated by cold-adapted microorganisms. To explore the natural enzyme diversity of these environments and identify new carboxylesterases, we haveExpand
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Identification and Characterization of Carboxyl Esterases of Gill Chamber-Associated Microbiota in the Deep-Sea Shrimp Rimicaris exoculata by Using Functional Metagenomics
ABSTRACT The shrimp Rimicaris exoculata dominates the fauna in deep-sea hydrothermal vent sites along the Mid-Atlantic Ridge (depth, 2,320 m). Here, we identified and biochemically characterizedExpand
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Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats.
The present study provides a deeper view of protein functionality as a function of temperature, salt and pressure in deep-sea habitats. A set of eight different enzymes from five distinct deep-seaExpand
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Exploring Bacterial Carboxylate Reductases for the Reduction of Bifunctional Carboxylic Acids.
Carboxylic acid reductases (CARs) selectively reduce carboxylic acids to aldehydes using ATP and NADPH as cofactors under mild conditions. Although CARs attracts significant interest, only a fewExpand
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Biochemical and Structural Studies of Uncharacterized Protein PA0743 from Pseudomonas aeruginosa Revealed NAD+-dependent l-Serine Dehydrogenase*
Background: β-Hydroxyacid dehydrogenases are ubiquitous enzymes, most of which remain uncharacterized. Results: Biochemical, crystallographic, and mutational analyses identified uncharacterizedExpand
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Structure and activity of the NAD(P)+‐dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium
Aldehyde dehydrogenases are found in all organisms and play an important role in the metabolic conversion and detoxification of endogenous and exogenous aldehydes. Genomes of many organisms includingExpand
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Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica.
The uncharacterized α/β-hydrolase protein OLEI01171 from the psychrophilic marine bacterium Oleispira antarctica belongs to the PF00756 family of putative esterases, which also includes humanExpand
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